采用酶动力学方法评估 [bmim][Cl] 离子液体在纤维素水解过程中对生物催化剂的抑制和失活作用。
Enzyme kinetics approach to assess biocatalyst inhibition and deactivation caused by [bmim][Cl] ionic liquid during cellulose hydrolysis.
机构信息
Research Institute on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem ut 10, 8200 Veszprém, Hungary.
Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, Bratislava 81237, Slovakia.
出版信息
Bioresour Technol. 2017 Apr;229:190-195. doi: 10.1016/j.biortech.2017.01.004. Epub 2017 Jan 7.
The aim of this work was to study the inhibition and deactivation of commercial enzyme cocktail (Cellic® Htec2) in the presence of [bmim][Cl] ionic liquid employing model cellulosic substrate, carboxymethyl cellulose (CMC). It turned out from the experiments - relying on enzyme kinetics approach - that [bmim][Cl] could act as a competitive inhibitor. Furthermore, depending on the process conditions i.e. contact of enzyme solution with high concentration [bmim][Cl], severe biocatalyst inactivation should be also taken into account as a potential risk during the enzymatic cellulose hydrolysis even in as short process times as few minutes.
本工作旨在研究在 [bmim][Cl] 离子液体存在下，使用模型纤维素底物羧甲基纤维素（CMC），对商业酶混合物（Cellic® Htec2）的抑制和失活作用。通过依赖于酶动力学方法的实验发现，[bmim][Cl] 可以作为一种竞争性抑制剂。此外，根据工艺条件，即在高浓度 [bmim][Cl] 存在下接触酶溶液，在酶解纤维素的过程中，即使在短短几分钟的过程时间内，也应考虑严重的生物催化剂失活，这是一个潜在的风险。
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