Yu Hak Sun, Park Mi-Kyung, Kang Shin Ae, Cho Kyu-Sup, Mun Sue Jean, Roh Hwan-Jung
Department of Parasitology, Pusan National University School of Medicine, Yangsan, 626-870, Republic of Korea.
Immunoregulatory Therapeutics Group in Brain Busan 21 project, Yangsan, Republic of Korea.
Stem Cell Res Ther. 2017 Jan 23;8(1):8. doi: 10.1186/s13287-016-0462-5.
In a previous study, we demonstrated that intravenous administration of adipose tissue stem cells (ASCs) could significantly reduce allergic symptoms and suppress eosinophilic inflammation.
To evaluate the secretome of ASCs, we administrated culture supernatant of ASCs (ASC sup, which contains the ASC secretome) and uncultured fresh medium (con sup) into a mouse model of allergic airway inflammation. Subsequently we observed the mice for signs of inflammation and investigated Th1-, Th2-, and T-related cytokine levels as well as recruitment of T cells into the airway.
We found that ASC sup could ameliorate allergic airway inflammation in this model; the value of airway hyperresponsiveness, and the occurrence of inflammatory cell infiltration in the lung, as well as the number of eosinophils, and goblet cells in the lung epithelium were all significantly decreased by ASC sup treatment. In addition, ASC sup treatment significantly decreased the levels of IL-4, IL-5, and IL-13 in the bronchial alveolar lavage fluid and in culture medium of lung-draining lymph node cells of the animal model of acute asthma. We detected numerous CTLA-4 and Foxp3-expressing cells in the lung after ASC sup treatment. ASC sup was found to have a higher concentration of IL-10 and TGF-β compared to con sup.
Stem cells have powerful potential for therapeutic functions in various diseases, but they also have many drawbacks. In this study, we found strong immunosuppressive ability of ASC sup in an allergic airway mouse model. It may be possible to use ASC sup for treatment of many immunological diseases in the near future.
在先前的一项研究中，我们证明静脉注射脂肪组织干细胞（ASC）可显著减轻过敏症状并抑制嗜酸性粒细胞炎症。
为评估ASC的分泌组，我们将ASC的培养上清液（ASC sup，其中含有ASC分泌组）和未培养的新鲜培养基（对照上清液）注入过敏性气道炎症小鼠模型。随后，我们观察小鼠的炎症迹象，并研究Th1、Th2和T相关细胞因子水平以及T细胞向气道的募集情况。
我们发现ASC sup可改善该模型中的过敏性气道炎症；ASC sup治疗可显著降低气道高反应性的值、肺部炎症细胞浸润的发生率以及肺上皮中嗜酸性粒细胞和杯状细胞的数量。此外，ASC sup治疗可显著降低急性哮喘动物模型支气管肺泡灌洗液和肺引流淋巴结细胞培养基中IL-4、IL-5和IL-13的水平。ASC sup治疗后，我们在肺中检测到大量表达CTLA-4和Foxp3的细胞。与对照上清液相比，发现ASC sup中IL-10和TGF-β的浓度更高。
干细胞在各种疾病中具有强大的治疗功能潜力，但也存在许多缺点。在本研究中，我们发现在过敏性气道小鼠模型中ASC sup具有强大的免疫抑制能力。在不久的将来，使用ASC sup治疗许多免疫性疾病可能是可行的。
