Xu Chong-feng, Duan Zi-yuan
Institute of Genetics and Developmental biology, Chinese Academy of Sciences,Beijing 100101, China.
Yi Chuan. 2017 Jan 20;39(1):75-86. doi: 10.16288/j.yczz.16-391.
A biorepository of human samples is essential to support the research of life science. Lymphoblastoid B cell line (LCL), which is easy to be prepared and can reproduce indefinitely, is a convenient form of sample preservation. LCLs are established from human B cells transformed by Epstein-Barr virus (EBV). Chinese National Immortalized Cell Bank has preserved human LCLs from different ethnic groups in China. As there are many studies on the nature of LCLs and public available resources with genome-wide data for LCLs, they have been widely applied in genetics, immunology, pharmacogenetics/genomics, regenerative medicine, cancer pathogenesis and immunotherapy, screening and generation of fully human neutralizing monoclonal antibodies and study on EBV pathogenesis. Here, we review the characteristics of LCLs and their contributions to scientific research, and introduce preserved samples in Chinese National Immortalized Cell Bank to the scientific community. We hope this bank can support more areas in the scientific research.
人类样本生物样本库对于支持生命科学研究至关重要。淋巴母细胞样B细胞系（LCL）易于制备且能无限增殖，是一种便捷的样本保存形式。LCL由爱泼斯坦-巴尔病毒（EBV）转化的人B细胞建立。中国国家永生细胞库保存了来自中国不同民族的人LCL。由于对LCL的性质有许多研究，且有LCL全基因组数据的公共可用资源，它们已广泛应用于遗传学、免疫学、药物遗传学/基因组学、再生医学、癌症发病机制与免疫治疗、全人源中和单克隆抗体的筛选与产生以及EBV发病机制研究。在此，我们综述LCL的特征及其对科研的贡献，并向科学界介绍中国国家永生细胞库中保存的样本。我们希望该样本库能支持科研的更多领域。
