Cell Engineering Division, RIKEN BioResource Center, Tsukuba, Ibaraki, Japan.
Genome Biol Evol. 2011;3:272-83. doi: 10.1093/gbe/evr014. Epub 2011 Mar 7.
The Sonoda-Tajima Cell Collection includes cell samples obtained from a range of ethnic minority groups across the world but in particular from South America. The collection is made all the more valuable by the fact that some of these ethnic populations have since died out, and thus it will be impossible to prepare a similar cell collection again. The collection was donated to our institute, a public cell bank in Japan, by Drs Sonoda and Tajima to make it available to researchers throughout the world. The original cell collection was composed of cryopreserved peripheral blood samples that would obviously have been rapidly exhausted if used directly. We, therefore, immortalized some samples with the Epstein-Barr virus and established B-lymphoblastoid cell lines (B-LCLs). As there is continuing controversy over whether the B-LCL genome is stably maintained, we performed an array comparative genomic hybridization (CGH) analysis to confirm the genomic stability of the cell lines. The array CGH analysis of the B-LCL lines and their parental B cells demonstrated that genomic stability was maintained in the long-term cell cultures. The B-LCLs of the Sonoda-Tajima Collection will therefore be made available to interested scientists around the world. At present, 512 B-LCLs have been developed, and we are willing to increase the number if there is sufficient demand.
索尼达-田岛细胞库包含了来自世界各地不同少数民族的细胞样本，尤其是来自南美洲的细胞样本。该细胞库的价值更高，因为其中一些少数民族已经灭绝，因此不可能再次制备类似的细胞库。该细胞库是由索尼达博士和田岛博士捐赠给我们研究所的，这是日本的一个公共细胞库，目的是向全世界的研究人员提供服务。原始的细胞库由冷冻保存的外周血样本组成，如果直接使用，这些样本显然会很快耗尽。因此，我们用 Epstein-Barr 病毒使一些样本永生化，并建立了 B 淋巴细胞母细胞系（B-LCL）。由于关于 B-LCL 基因组是否稳定维持的问题仍存在争议，我们进行了 array 比较基因组杂交（CGH）分析以确认细胞系的基因组稳定性。B-LCL 系及其亲本 B 细胞的 array CGH 分析表明，在长期的细胞培养中保持了基因组稳定性。因此，索尼达-田岛细胞库的 B-LCL 将提供给全世界感兴趣的科学家。目前，已经开发了 512 个 B-LCL，我们愿意根据需求增加数量。
