基于单克隆抗体的免疫荧光检测法检测血培养中伯克霍尔德菌的验证。
Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures.
机构信息
Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
出版信息
Trans R Soc Trop Med Hyg. 2016 Nov 1;110(11):670-672. doi: 10.1093/trstmh/trw079.
Abstract
BACKGROUND
Identification of Burkholderia pseudomallei, the cause of melioidosis, using routine methods takes several days. Use of a monoclonal antibody-based immunofluorescent assay (IFA) on positive blood cultures may speed diagnosis.
METHODS
We tested the diagnostic accuracy of the IFA on 545 blood cultures positive for Gram-negative organisms at Udon Thani Hospital, Thailand, between June 2015 and August 2016.
RESULTS
Sensitivity of the IFA was 100% and specificity was 99.6%. The median decrease in time to pathogen identification between the IFA result and routine methods was 28 h (IQR 25-51), p<0.0001.
CONCLUSIONS
The IFA accurately expedites the diagnosis of melioidosis.
摘要
背景
使用常规方法鉴定导致类鼻疽的伯克霍尔德菌需要数天时间。在阳性血培养物上使用基于单克隆抗体的免疫荧光检测（IFA）可能会加速诊断。
方法
我们在 2015 年 6 月至 2016 年 8 月期间，在泰国乌隆他尼医院检测了 545 份革兰氏阴性菌阳性血培养物的 IFA 的诊断准确性。
结果
IFA 的敏感性为 100%，特异性为 99.6%。IFA 结果与常规方法相比，鉴定病原体的时间中位数减少了 28 小时（IQR 25-51），p<0.0001。
结论
IFA 可准确快速诊断类鼻疽。
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本文引用的文献
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Am J Trop Med Hyg. 2013 Nov;89(5):971-972. doi: 10.4269/ajtmh.13-0212. Epub 2013 Sep 9.
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Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of Burkholderia pseudomallei in clinical specimens.基于单克隆抗体的免疫荧光显微镜快速鉴定临床标本中的鼻疽伯克霍尔德菌。
Am J Trop Med Hyg. 2013 Jul;89(1):165-168. doi: 10.4269/ajtmh.13-0066. Epub 2013 May 28.
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