Cao Tinghui, He Min, Bai Tianyu, Liu Hui
College of Medical Laboratory, Dalian Medical University, Dalian, China.
College of Medical Laboratory, Dalian Medical University, Dalian, China; Department of Clinical Laboratory, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, China.
Bioinorg Chem Appl. 2016;2016:7054049. doi: 10.1155/2016/7054049. Epub 2016 Dec 27.
. To establish a new method for determination of antioxidant capacity of human urine based on the redox couple I/KI and to evaluate the redox status of healthy and diseased individuals. . The method was based on the linear relationship between oxidation reduction potential (ORP) and logarithm of concentration ratio of I/KI. ORP of a solution with a known concentration ratio of I/KI will change when reacted with urine. To determine the accuracy of the method, both vitamin C and urine were reacted separately with I/KI solution. The new method was compared with the traditional method of iodine titration and then used to measure the antioxidant capacity of urine samples from 30 diabetic patients and 30 healthy subjects. . A linear relationship was found between logarithm of concentration ratio of I/KI and ORP ( = 0.998). Both vitamin C and urine concentration showed a linear relationship with ORP ( = 0.994 and 0.986, resp.). The precision of the method was in the acceptable range and results of two methods had a linear correlation ( = 0.987). Differences in ORP values between diabetic group and control group were statistically significant ( < 0.05). . A new method for measuring the antioxidant capacity of clinical urine has been established.
建立一种基于I/KI氧化还原对测定人尿抗氧化能力的新方法，并评估健康个体和患病个体的氧化还原状态。该方法基于氧化还原电位（ORP）与I/KI浓度比对数之间的线性关系。当与尿液反应时，具有已知I/KI浓度比的溶液的ORP会发生变化。为了确定该方法的准确性，分别将维生素C和尿液与I/KI溶液反应。将该新方法与传统的碘滴定法进行比较，然后用于测量30例糖尿病患者和30例健康受试者尿液样本的抗氧化能力。发现I/KI浓度比的对数与ORP之间存在线性关系（=0.998）。维生素C和尿液浓度与ORP均呈线性关系（分别为=0.994和0.986）。该方法的精密度在可接受范围内，两种方法的结果具有线性相关性（=0.987）。糖尿病组和对照组之间的ORP值差异具有统计学意义（<0.05）。已建立一种测量临床尿液抗氧化能力的新方法。
