Shnaiderman-Torban Anat, Paitan Yossi, Arielly Haia, Kondratyeva Kira, Tirosh-Levy Sharon, Abells-Sutton Gila, Navon-Venezia Shiri, Steinman Amir
Koret School of Veterinary Medicine (KSVM), The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 761001, Israel.
Department of Clinical Microbiology and Immunology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 6997801, Israel.
Animals (Basel). 2019 Aug 23;9(9):600. doi: 10.3390/ani9090600.
Extended-spectrum β-lactamase (ESBL-E) have been investigated in adult horses, but not in foals. We aimed to determine shedding and infection in neonatal foals and mares. Rectal swabs were sampled from mare and foal pairs on admission and on the 3rd day of hospitalization; enriched, plated, and bacteria were verified for ESBL production. Identification and antibiotic susceptibility profiles were determined (Vitek2). Genotyping was performed by multilocus sequence typing (MLST). Genes were identified by PCR and Sanger sequencing. Medical data were analyzed for risk factors (SPSS). On admission, 55 pairs were sampled, of which 33 pairs were re-sampled. Shedding rates on admission in foals and mares were 33% (95% CI 21-47%) and 16% (95% CI 8-29%), respectively, and during hospitalization, these increased significantly to 85% (95% CI 70-94%) and 58% (95% CI 40-73%), respectively. Foal shedding was associated with umbilical infection on admission ( = 0.016) and with ampicillin treatment during hospitalization ( = 0.011), and was independent of the mare's shedding. The most common ESBL-E was . During hospitalization, species diversity increased. Four foals were infected with ESBL-E strains, including umbilical infections and wounds. This study substantiates an alarming prevalence of shedding in neonatal foals, which should be further investigated in order to reduce resistance rates.
超广谱β-内酰胺酶（ESBL-E）已在成年马匹中进行了研究，但尚未在幼驹中进行研究。我们旨在确定新生幼驹和母马的细菌排泄情况和感染情况。在入院时和住院第3天采集母马和幼驹对的直肠拭子；进行富集、接种培养，并验证细菌是否产生ESBL。确定细菌的鉴定结果和抗生素敏感性谱（Vitek2系统）。通过多位点序列分型（MLST）进行基因分型。通过聚合酶链反应（PCR）和桑格测序鉴定基因。分析医疗数据以确定危险因素（SPSS软件）。入院时采集了55对样本，其中33对进行了再次采样。幼驹和母马入院时的细菌排泄率分别为33%（95%置信区间21%-47%）和16%（95%置信区间8%-29%），在住院期间，这些比率分别显著增加至85%（95%置信区间70%-94%）和58%（95%置信区间40%-73%）。幼驹的细菌排泄与入院时的脐部感染（P = 0.016）以及住院期间的氨苄西林治疗（P = 0.011）有关，并且与母马的细菌排泄无关。最常见的ESBL-E是[具体菌种未给出]。在住院期间，菌种多样性增加。4匹幼驹感染了ESBL-E菌株，包括脐部感染和伤口感染。本研究证实了新生幼驹中细菌排泄的惊人患病率，应进一步调查以降低耐药率。
