Tušek Ana Jurinjak, Šalić Anita, Zelić Bruno
Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000, Zagreb, Croatia.
Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000, Zagreb, Croatia.
Appl Biochem Biotechnol. 2017 Aug;182(4):1575-1590. doi: 10.1007/s12010-017-2419-2. Epub 2017 Jan 23.
Laccase belongs to the group of enzymes that are capable to catalyze the oxidation of phenols. Since the water is only by-product in laccase-catalyzed phenol oxidations, it is ideally "green" enzyme with many possible applications in different industrial processes. To make the oxidation process more sustainable in terms of biocatalyst consumption, immobilization of the enzyme is implemented in to the processes. Additionally, when developing a process, choice of a reactor type plays a significant role in the total outcome.In this study, the use of immobilized laccase from Trametes versicolor for biocatalytic catechol oxidation was explored. Two different methods of immobilization were performed and compared using five different reactor types. In order to compare different systems used for catechol oxidation, biocatalyst turnover number and turnover frequency were calculated. With low consumption of the enzyme and good efficiency, obtained results go in favor of microreactors with enzyme covalently immobilized on the microchannel surface.
漆酶属于能够催化酚类氧化的酶类。由于在漆酶催化的酚类氧化反应中，水只是副产物，因此它是一种理想的“绿色”酶，在不同的工业过程中有许多潜在应用。为了使氧化过程在生物催化剂消耗方面更具可持续性，酶的固定化被应用于这些过程中。此外，在开发一个过程时，反应器类型的选择对总体结果起着重要作用。在本研究中，探索了使用来自云芝的固定化漆酶进行生物催化邻苯二酚氧化。采用了两种不同的固定化方法，并使用五种不同的反应器类型进行比较。为了比较用于邻苯二酚氧化的不同系统，计算了生物催化剂的周转数和周转频率。由于酶消耗低且效率高，所得结果表明将酶共价固定在微通道表面的微反应器更具优势。
