用于研究血管紧张素 -（1 - 7）作用的离体主动脉血管反应性

Vascular Reactivity of Isolated Aorta to Study the Angiotensin-(1-7) Actions.

作者信息

Lautner Roberto Q, Fraga-Silva Rodrigo A, Ferreira Anderson J, Santos Robson A S

机构信息

National Institute of Science and Technology in Nanobiopharmaceutics, Gerais, Brazil.

Department of Physiology and Biophysics, Biological Science Institute, Federal University of Minas Gerais, Av. Pres. Antonio Carols 6627, Pampulha, Belo Horizonte, MG, 31270-901, Brazil.

出版信息

Methods Mol Biol. 2017;1527:369-379. doi: 10.1007/978-1-4939-6625-7_29.

DOI:10.1007/978-1-4939-6625-7_29

PMID:28116731

Abstract

Since the early 1950s, vascular reactivity using isolated vessel rings has been a useful and efficient model for physiological and pharmacological studies. This experimental model was utilized in the milestone study of Dr. Robert Furchgott to discover the endothelium-derived relaxation factor (EDRF) nitric oxide (NO), opening new avenues and scientific perspectives in the vascular pathophysiology. Moreover, the isolated vessel ring preparation had an important contribution to understand many vasoactive systems. Here, we described the isolated aorta technique and pitfalls about the use of angiotensin-(1-7) peptide in this preparation.

摘要

自20世纪50年代初以来，使用离体血管环研究血管反应性一直是生理和药理研究中一种有用且高效的模型。罗伯特·弗奇戈特博士的里程碑式研究就利用了这个实验模型来发现内皮源性舒张因子（EDRF）一氧化氮（NO），为血管病理生理学开辟了新的途径和科学视角。此外，离体血管环制备对于理解许多血管活性系统也有重要贡献。在此，我们描述了离体主动脉技术以及在该制备中使用血管紧张素 -（1 - 7）肽的注意事项。