Leal Marcos André Soares, de Almeida Thanisia, Torres João Guilherme, Gastalho Campos Luciene Cristina, Vasquez Elisardo Corral, Barauna Valério Garrone
Laboratory of Translational Physiology, Federal University of Espirito, Vitória, Espirito Santo, Brazil.
Laboratory of Molecular Physiology, Federal University of Espírito Santo, Vitória, Espirito Santo, Brazil.
Adv Pharmacol Pharm Sci. 2020 Feb 21;2020:3092721. doi: 10.1155/2020/3092721. eCollection 2020.
Vascular reactivity experiments using isolated aortic rings have been widely used as a model for physiological and pharmacological studies since the early sixties. Here, we suggest several parameters that the researcher should pay attention to when investigating angiotensin II in their experimental models. Angiotensin II is one of the active peptides of the renin-angiotensin system and exerts its effect through the AT1 and AT2 receptors. Some studies seek to understand the effects of angiotensin II receptors at the vascular level by using vascular reactivity experiments. However, because of the large number of variations, there are only a handful of reactivity studies that seek to use this method. Thus, the objective of this study was to standardize experimental methods with angiotensin II, through vascular reactivity protocols. For this, variables such as basal tension, concentration interval, single concentration, curve concentration response, and multiple experiments using the same aortic ring were developed using the technique of vascular reactivity in an organ bath. This is the first study that has standardized the vascular reactivity protocol. In addition, we demonstrated the effects of TRV023-biased ligand of the AT1R at vascular sites.
自20世纪60年代初以来，使用离体主动脉环进行的血管反应性实验已被广泛用作生理学和药理学研究的模型。在此，我们提出了几个研究人员在其实验模型中研究血管紧张素II时应注意的参数。血管紧张素II是肾素-血管紧张素系统的活性肽之一，通过AT1和AT2受体发挥作用。一些研究试图通过血管反应性实验来了解血管紧张素II受体在血管水平的作用。然而，由于存在大量变量，只有少数反应性研究试图使用这种方法。因此，本研究的目的是通过血管反应性方案来规范血管紧张素II的实验方法。为此，利用器官浴中的血管反应性技术，开发了诸如基础张力、浓度区间、单一浓度、曲线浓度反应以及使用同一主动脉环进行多次实验等变量。这是第一项对血管反应性方案进行标准化的研究。此外，我们还证明了AT1R的TRV023偏向配体在血管部位的作用。
