Usa Kristie S, Liu Yong, Kurth Terry, Kriegel Alison J, Mattson David L, Cowley Allen W, Liang Mingyu
Center of Systems Molecular Medicine, Department of Physiology, Medical College of Wisconsin, 8701 Watertown PlankRoad, Milwaukee, WI, 53226, USA.
Methods Mol Biol. 2017;1527:409-419. doi: 10.1007/978-1-4939-6625-7_31.
MicroRNAs are endogenous small, non-protein-coding RNA molecules that play an important role in the regulation of a wide variety of cellular functions and disease processes. A novel role for microRNAs in the development of hypertension and hypertensive tissue injury is emerging in recent studies. Development of hypertension involves multiple organ systems and cannot be modeled in vitro. Therefore, the ability to experimentally alter genes, gene products, or biological pathways, including microRNAs, in an organ-specific manner in intact animal models is particularly valuable to hypertension research. The kidney plays a central role in the long-term regulation of arterial blood pressure. In this chapter, we describe a detailed protocol for using a renal interstitial injection method to deliver anti-miR oligonucleotides to knock down microRNA specifically in the kidney in conscious rats.
微小RNA是内源性的小的非蛋白质编码RNA分子，在多种细胞功能和疾病过程的调节中发挥重要作用。最近的研究中出现了微小RNA在高血压发展和高血压组织损伤中的新作用。高血压的发展涉及多个器官系统，无法在体外进行模拟。因此，在完整动物模型中以器官特异性方式实验性改变基因、基因产物或生物途径（包括微小RNA）的能力对高血压研究特别有价值。肾脏在动脉血压的长期调节中起核心作用。在本章中，我们描述了一种详细的方案，用于使用肾间质注射方法将抗微小RNA寡核苷酸递送至清醒大鼠的肾脏中，以特异性敲低微小RNA。
