Ovesen Sandra, Kirk Karina Frahm, Nielsen Hans Linde, Nielsen Henrik
Department of Infectious Diseases, Aalborg University Hospital, Aalborg, Denmark.
Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
APMIS. 2017 Mar;125(3):230-235. doi: 10.1111/apm.12655. Epub 2017 Jan 24.
Campylobacter concisus is an emerging pathogen associated with gastrointestinal disorders such as gastroenteritis and inflammatory bowel diseases (IBD), but the species is also found in healthy subjects. The heterogeneous genome of C. concisus increases the likelihood of varying virulence between strains. Flagella motility is a crucial virulence factor for the well-recognized Campylobacter jejuni; therefore, this study aimed to analyze the motility of C. concisus isolated from saliva, gut biopsies, and feces of patients with IBD, gastroenteritis, and healthy subjects. The motility zones of 63 isolates from 52 patients were measured after microaerobic growth in soft-agar plates for 72 hours. The motility of C. concisus was significantly lower than that of Campylobacter jejuni and Campylobacter fetus subsp. fetus. The motility of C. concisus varied between isolates (4-22 mm), but there was no statistical significant difference between isolates from IBD patients and healthy subjects (p = 0.14). A tendency of a larger motility zones was observed for IBD gut mucosa isolates, although it did not reach statistical significance (p = 0.13), and no difference was found between oral or fecal isolates between groups. In conclusion, the varying motility of C. concisus could not be related to disease outcome or colonization sites.
简明弯曲杆菌是一种新出现的病原体，与诸如肠胃炎和炎症性肠病（IBD）等胃肠道疾病相关，但在健康个体中也能发现该菌种。简明弯曲杆菌的基因组具有异质性，这增加了不同菌株之间毒力各异的可能性。鞭毛运动性是公认的空肠弯曲杆菌的关键毒力因子；因此，本研究旨在分析从IBD患者、肠胃炎患者及健康受试者的唾液、肠道活检组织和粪便中分离出的简明弯曲杆菌的运动性。在软琼脂平板上微需氧培养72小时后，测量了来自52名患者的63株分离菌的运动区域。简明弯曲杆菌的运动性显著低于空肠弯曲杆菌和胎儿弯曲杆菌胎儿亚种。简明弯曲杆菌不同分离菌的运动性有所不同（4 - 22毫米），但IBD患者分离菌与健康受试者分离菌之间无统计学显著差异（p = 0.14）。尽管未达到统计学显著水平（p = 0.13），但观察到IBD肠道黏膜分离菌有运动区域更大的趋势，且两组之间口腔或粪便分离菌无差异。总之，简明弯曲杆菌运动性的差异与疾病结局或定植部位无关。
