Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, Bhabha Atomic Research Centre, Kalpakkam 603 102, Tamil Nadu, India.
Biofouling and Biofilm Processes Section, Water and Steam Chemistry Division, Bhabha Atomic Research Centre, Kalpakkam 603 102, Tamil Nadu, India; Homi Bhabha National Institute, Mumbai 400 094, India.
Mar Pollut Bull. 2017 Nov 30;124(2):819-826. doi: 10.1016/j.marpolbul.2016.12.059. Epub 2017 Jan 20.
Phytoplankton entrained into cooling water systems of coastal power stations are subjected to acute chemical stress due to biocides (chlorine) used for biofouling control. They are subsequently released into the environment, where they may survive/recover or succumb. Experiments were conducted to evaluate the susceptibility of a centric (Chaetoceros lorenzianus) and pennate (Navicula sp.) diatom to in-plant administered concentrations of chlorine (0.2-0.5mg/L, TRO). Viability of cells exposed to chlorine was assessed by SYTOX® Green fluorimetry and was compared with other conventional end points like total cell counts, chlorophyll a content and cellular autofluorescence. Results showed a concentration-dependant reduction in viability, chlorophyll a and autofluorescence. C. lorenzianus cells were more susceptible to chlorine compared to Navicula sp. SYTOX® Green staining appears to be a sensitive method to assess chlorine-induced damages. The data show that in-use levels of chlorination can potentially impact entrained organisms; however, they can recover when returned to coastal waters.
海水中的浮游植物被带入沿海电站的冷却水中，由于使用杀生剂（氯）来控制生物污垢，它们会受到急性化学胁迫。随后它们被释放到环境中，在那里它们可能会存活/恢复或死亡。实验旨在评估一种有孔虫（Chaetoceros lorenzianus）和舟形藻（Navicula sp.）硅藻对工厂内施加的氯浓度（0.2-0.5mg/L，TRO）的敏感性。通过 SYTOX® Green 荧光法评估暴露于氯的细胞的活力，并将其与其他常规终点（如总细胞计数、叶绿素 a 含量和细胞自发荧光）进行比较。结果表明，细胞活力、叶绿素 a 和自发荧光均随浓度的增加而降低。与 Navicula sp. 相比，C. lorenzianus 细胞对氯更敏感。SYTOX® Green 染色似乎是一种评估氯诱导损伤的敏感方法。数据表明，实际使用的氯化水平可能会对带入的生物产生影响；然而，当它们返回沿海水域时，它们可以恢复。
