Indrayoni Putu, Purwanti Diah Intan, Wongso Suwidji, Prajogo Bambang E W, Indrayanto Gunawan
Faculty of Pharmacy, Airlangga University, Jl. Dharmawangsa Dalam, Surabaya 60286, Indonesia.
PT. Angler BioChemLab, Plaza Graha Family C-25, Surabaya 60226, Indonesia.
Sci Pharm. 2016 Apr 13;84(3):555-566. doi: 10.3390/scipharm84030555.
Metabolite profiles of plant organs and their in vitro cultures of have been studied by using Ultra Performance Liquid Chromatography-Quadrupole Time-of-Flight-Mass Spectrometry (UPLC-Qtof-MS). Samples of leaves, stems, roots, and shoot cultures showed similar patterns of metabolites, while samples of root cultures gave very different profiles. Concentrations of secondary metabolites in shoot cultures were relatively low compared to those in the leaves and roots of the plants. The results suggested that secondary metabolites in were biosynthetized in the leaves, then transported to the roots.
利用超高效液相色谱-四极杆飞行时间质谱(UPLC-Qtof-MS)研究了植物器官及其离体培养物的代谢物谱。叶片、茎、根和茎尖培养物的样品显示出相似的代谢物模式,而根培养物的样品则呈现出非常不同的谱图。与植物叶片和根中的次生代谢物浓度相比,茎尖培养物中的次生代谢物浓度相对较低。结果表明,[植物名称未给出]中的次生代谢物在叶片中生物合成,然后运输到根部。