IN SILICO SCREENING AND BIOLOGICAL EVALUATION OF THE COMPOUNDS OF LEAVES EXTRACT AS INTERFERON GAMMA INDUCER: A STUDY OF ANTI HUMAN IMMUNODEFICIENCY VIRUS (HIV) DEVELOPMENT.

BACKGROUND

Burm f. (Achanthaceae) has been known as traditional medicine in Indonesia. It contains of flavonoids and alkaloids. This study was conducted to evaluate the effect of on the profile of IFN-γ on mice ). Molecular docking test was also conducted to determine the interaction of alkaloids and flavonoids on the leaves against IFN-γ receptor. It is expected that this research will provide scientific information on the development of leaves as an anti-HIV drug.

MATERIALS AND METHODS

The molecular docking test was performed by using Molegro Virtual Docker software to predict the interaction of alkaloid and flavonoid compounds of leaves with IFN-γ receptor. In the in vivo test, the effects of 70% ethanol extract, fractionated 70% ethanol extract, and water extract of leaves were evaluated on the profile of IFN-γ stimulation on mice (. The test was performed by administering the three gendarussa extracts into the nine groups of mice for 14 days.

RESULTS

Based on the molecular docking test, it was found that flavonoid of leaves have lower effects on the IFN-γ receptor than the alkaloids. From the in vivo test on mice, it was found that the fractionated 70% ethanol extract of leaves did not induce the level of IFN-γ. On the other hand, both 70% ethanol and water extract of leaves induced the production of IFN-γ.

CONCLUSION

Fractionated 70% ethanol extract of does not induce the production of IFN-γ, so it can be developed as anti HIV drugs.