XLIM Research Institute, UMR CNRS No 7252, University of Limoges, Faculty of Science and Techniques, 123 Avenue Albert Thomas, 87060 Limoges, France.
Sci Rep. 2017 Jan 24;7:41267. doi: 10.1038/srep41267.
High powered, nanosecond duration, pulsed electric fields (nsPEF) cause cell death by a mechanism that is not fully understood and have been proposed as a targeted cancer therapy. Numerous chemotherapeutics work by disrupting microtubules. As microtubules are affected by electrical fields, this study looks at the possibility of disrupting them electrically with nsPEF. Human glioblastoma cells (U87-MG) treated with 100, 10 ns, 44 kV/cm pulses at a frequency of 10 Hz showed a breakdown of their interphase microtubule network that was accompanied by a reduction in the number of growing microtubules. This effect is temporally linked to loss of mitochondrial membrane potential and independent of cellular swelling and calcium influx, two factors that disrupt microtubule growth dynamics. Super-resolution microscopy revealed microtubule buckling and breaking as a result of nsPEF application, suggesting that nsPEF may act directly on microtubules.
高功率、纳秒持续时间的脉冲电场 (nsPEF) 通过一种尚未完全了解的机制导致细胞死亡，并已被提议作为一种靶向癌症治疗方法。许多化疗药物通过破坏微管起作用。由于微管受电场影响，本研究探讨了用 nsPEF 电破坏它们的可能性。用 100、10ns、44kV/cm 的脉冲频率为 10Hz 处理人神经胶质瘤细胞 (U87-MG) 后，其有丝分裂微管网络破裂，同时生长中的微管数量减少。这种效应与线粒体膜电位丧失有关，与细胞肿胀和钙离子内流无关，这两个因素会破坏微管生长动力学。超分辨率显微镜显示 nsPEF 应用导致微管弯曲和断裂，表明 nsPEF 可能直接作用于微管。
