Szulc Justyna, Otlewska Anna, Okrasa Małgorzata, Majchrzycka Katarzyna, Sulyok Michael, Gutarowska Beata
Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Łódź 90-924, Poland.
Department of Personal Protective Equipment, Central Institute for Labour Protection, National Research Institute, Łódź 90-133, Poland.
Int J Environ Res Public Health. 2017 Jan 21;14(1):99. doi: 10.3390/ijerph14010099.
The aim of the study was to evaluate the microbial contamination at a plant biomass processing thermal power station (CHP). We found 2.42 × 10³ CFU/m³ of bacteria and 1.37 × 10⁴ CFU/m³ of fungi in the air; 2.30 × 10⁷ CFU/g of bacteria and 4.46 × 10⁵ CFU/g of fungi in the biomass; and 1.61 × 10² CFU/cm² bacteria and 2.39 × 10¹ CFU/cm² fungi in filtering facepiece respirators (FFRs). Using culture methods, we found 8 genera of mesophilic bacteria and 7 of fungi in the air; 10 genera each of bacteria and fungi in the biomass; and 2 and 5, respectively, on the FFRs. Metagenomic analysis (Illumina MiSeq) revealed the presence of 46 bacterial and 5 fungal genera on the FFRs, including potential pathogens , , sp., sp., sp.). The ability of microorganisms to create a biofilm on the FFRs was confirmed using scanning electron microscopy (SEM). We also identified secondary metabolites in the biomass and FFRs, including fumigaclavines, quinocitrinines, sterigmatocistin, and 3-nitropropionic acid, which may be toxic to humans. Due to the presence of potential pathogens and mycotoxins, the level of microbiological contamination at workplaces in CHPs should be monitored.
本研究的目的是评估一家植物生物质加工热电厂（CHP）的微生物污染情况。我们发现空气中细菌含量为2.42×10³CFU/m³，真菌含量为1.37×10⁴CFU/m³；生物质中细菌含量为2.30×10⁷CFU/g，真菌含量为4.46×10⁵CFU/g；过滤式面罩呼吸器（FFR）上细菌含量为1.61×10²CFU/cm²，真菌含量为2.39×10¹CFU/cm²。采用培养方法，我们发现空气中有8个嗜温细菌属和7个真菌属；生物质中有10个细菌属和10个真菌属；FFR上分别有2个和5个。宏基因组分析（Illumina MiSeq）显示FFR上存在46个细菌属和5个真菌属，包括潜在病原体（ 、 、 属、 属、 属）。使用扫描电子显微镜（SEM）证实了微生物在FFR上形成生物膜的能力。我们还在生物质和FFR中鉴定出次生代谢产物，包括烟曲霉毒素、醌柑橘素、柄曲霉素和3-硝基丙酸，这些可能对人类有毒。由于存在潜在病原体和霉菌毒素，热电厂工作场所的微生物污染水平应受到监测。
