Wang Dong, Tediashvili Grigol, Pecha Simon, Reichenspurner Hermann, Deuse Tobias, Schrepfer Sonja
Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg; Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF); Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck; Cardiovascular Surgery, University Heart Center Hamburg.
Transplant and Stem Cell Immunobiology Lab, University Heart Center Hamburg; Department of Surgery, Transplant and Stem Cell Immunobiology Lab, University of California San Francisco (UCSF); Cardiovascular Research Center (CVRC) and DZHK German Center for Cardiovascular Research), partner site Hamburg/Kiel/Luebeck.
J Vis Exp. 2017 Jan 15(119):54839. doi: 10.3791/54839.
Bypass grafting is an established treatment method for coronary artery disease. Graft patency continues to be the Achilles heel of saphenous vein grafts. Research models for bypass graft failure are essential for a better understanding of pathobiological and pathophysiological processes during graft patency loss. Large animal models, such as pigs or sheep, resemble human anatomical structures but require special facilities and equipment. This video describes a rat vein interposition model to investigate vein graft patency loss. Rats are inexpensive and easy to handle. Compared to mouse models, the convenient size of rats permits better operability and enables a sufficient amount of material to be obtained for further diverse analysis. In brief, the inferior epigastric vein of a donor rat is harvested and used to replace a segment of the femoral artery. Anastomosis is conducted via single stitches and sealed with fibrin glue. Graft patency can be monitored non-invasively using duplex sonography. Myointimal hyperplasia, which is the main cause for graft patency loss, develops progressively over time and can be calculated from histological cross sections.
旁路移植术是治疗冠状动脉疾病的一种成熟方法。移植血管通畅性仍然是大隐静脉移植的致命弱点。旁路移植失败的研究模型对于更好地理解移植血管通畅性丧失过程中的病理生物学和病理生理过程至关重要。大型动物模型，如猪或羊，与人体解剖结构相似，但需要特殊的设施和设备。本视频介绍了一种用于研究静脉移植血管通畅性丧失的大鼠静脉移植模型。大鼠价格低廉且易于操作。与小鼠模型相比，大鼠合适的大小便于操作，并且能够获得足够的材料用于进一步的多样分析。简而言之，获取供体大鼠的腹壁下静脉，并用其替换一段股动脉。通过单针缝合进行吻合，并用纤维蛋白胶密封。可使用双功超声对移植血管通畅性进行无创监测。肌内膜增生是移植血管通畅性丧失的主要原因，其会随时间逐渐发展，可从组织学横截面计算得出。
