Anhui Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, PR China.
Department of Cardiac Surgery, Anhui Provincial Hospital, Anhui Medical University, Hefei, PR China.
Cell Transplant. 2020 Jan-Dec;29:963689720969173. doi: 10.1177/0963689720969173.
Coronary artery bypass grafting (CABG) is still the most effective method for the treatment of coronary heart disease at present. However, the restenosis of vein grafts following surgery is an important complication of CABG. In this study, polysaccharide (BSP), which has anti-inflammatory and antiproliferative properties, was used to prevent or delay the proliferation of venous bridge endothelial cells in a rat model. We transplanted the autogenous jugular vein to the rat carotid artery, and wrapped it with BSP. We carried out experiments in 4 groups (with 24 rats in each group): a high-BSP dose group (the HBG group, 10 mg), a low-BSP dose group (the LBG group, 3 mg), a pluronic gel group (the gel group), and a control group. Vein grafts were then harvested after 3, 14, and 28 days. Following transplantation, we used color Doppler ultrasound to assess the patency of the transplanted vein. The grafted veins were stained with hematoxylin and eosin (H&E) and Masson to measure the thickness of the intima and media of the blood vessels. Proliferating cell nuclear antigen (PCNA) and vascular cell adhesion molecule-l (VCAM-1) were assessed in vein grafts by immunohistochemistry and western blotting. We detected a significant reduction in the proliferation of endothelial cells in the BSP group compared with the control group ( < 0.05). H&E and Masson's trichrome staining showed that the extent of intimal hyperplasia in transplanted veins from the high BSP group (HBS) (67.42 ± 0.54 µm) and low BSP group (LBS) (120.83 ± 1.87 µm) groups was significantly lower than that in the control group (257.03 ± 2.74 µm, < 0.05), and that the extent of intimal hyperplasia in the HBS group was lower than that in the LBS group ( < 0.05). We found that the effect of BSP was dose-dependent, as high-dose BSP had a more significant inhibitory effect on cell proliferation than low-dose BSP ( < 0.05). The results of immunohistochemistry and western blotting showed that PCNA and VCAM-1 were significantly downregulated in the BSP treatment group on days 14 and 28 ( < 0.05). BSP inhibits the proliferation of vascular endothelial cells and reduces the expression of VCAM-1, thereby inhibiting the restenosis of graft veins.
冠状动脉旁路移植术（CABG）仍然是目前治疗冠心病最有效的方法。然而，手术后静脉移植物的再狭窄是 CABG 的一个重要并发症。在这项研究中，具有抗炎和抗增殖特性的多糖（BSP）被用于预防或延迟大鼠模型静脉桥内皮细胞的增殖。我们将自体颈静脉移植到大鼠颈动脉中，并包裹 BSP。我们在 4 组（每组 24 只大鼠）中进行了实验：高 BSP 剂量组（HBG 组，10mg）、低 BSP 剂量组（LBG 组，3mg）、泊洛沙姆凝胶组（凝胶组）和对照组。然后在 3、14 和 28 天后收获静脉移植物。移植后，我们使用彩色多普勒超声评估移植静脉的通畅性。用苏木精和伊红（H&E）和 Masson 染色测量血管内膜和中膜的厚度。通过免疫组化和 Western blot 评估静脉移植物中增殖细胞核抗原（PCNA）和血管细胞黏附分子-1（VCAM-1）的表达。与对照组相比，BSP 组内皮细胞的增殖明显减少（<0.05）。H&E 和 Masson 三色染色显示，高 BSP 组（HBS）（67.42±0.54μm）和低 BSP 组（LBS）（120.83±1.87μm）静脉移植物的内膜增生程度明显低于对照组（257.03±2.74μm，<0.05），HBS 组的内膜增生程度低于 LBS 组（<0.05）。我们发现 BSP 的作用呈剂量依赖性，高剂量 BSP 对细胞增殖的抑制作用强于低剂量 BSP（<0.05）。免疫组化和 Western blot 结果显示，BSP 处理组在 14 天和 28 天 PCNA 和 VCAM-1 的表达明显下调（<0.05）。BSP 抑制血管内皮细胞的增殖，降低 VCAM-1 的表达，从而抑制移植物静脉的再狭窄。
