Perazzi Anna, Bonsembiante Federico, Gelain Maria Elena, Patruno Marco, Di Iorio Enzo, Migliorati Angelo, Iacopetti Ilaria
Department of Animal Medicine, Production and Health, University of Padua, Padua, Italy.
Department of Comparative Biomedicine and Food Science, University of Padua, Padua, Italy.
Vet Clin Pathol. 2017 Mar;46(1):164-171. doi: 10.1111/vcp.12450. Epub 2017 Jan 24.
Impression cytology (IC) is a noninvasive technique in which filters are used to sample superficial layers of ocular epithelium.
The aim of this study was to compare cytology specimens obtained by IC and cytobrush from healthy canine and feline eyes.
Dogs and cats were prospectively sampled using polytetrafluorethylene filters on the right eye, and cytobrush on the left eye. Wright-Giemsa-stained specimens were evaluated by 2 observers. Cellularity, preservation, and morphology of cells and presence of goblet and inflammatory cells were scored with a 4-grade scale. Inter-observer agreement and effects of topical anesthesia were analyzed.
In 20 canine IC samples, 10 showed good cellularity (score 2-3) and 13 good preservation. Superficial epithelial cells (SEC) were present in 13/20 of IC, while basal-intermediate cells (BIC) were seen in 14/20. In 6/20 and 7/20, goblet and inflammatory cells were noted, respectively. In 20 cats, 15 of IC showed good cellularity and 14 good preservation, and SEC were present in 16/20 of IC and BIC in 17/20. In 13/20 and 3/20 cats, goblet cells and inflammatory cells were noted, respectively. Canine cytobrush specimens appeared well preserved (9/20) and had good cellularity (8/20). In feline cytobrush specimens, good preservation and cellularity were observed in 16/20 and 14/20, respectively. In both species, all cell types were present without a clear separation. There was moderate to fair agreement about cellular morphology in IC between observers. Specimens obtained with and without anesthesia were comparable.
Impression cytology allowed collection of samples with maintained cytoarchitecture, while cytoplasmatic and nuclear details were often difficult to evaluate.
印迹细胞学检查（IC）是一种非侵入性技术，使用滤器对眼表上皮浅层进行采样。
本研究旨在比较通过IC和细胞刷从健康犬猫眼中获取的细胞学标本。
对犬猫进行前瞻性采样，右眼使用聚四氟乙烯滤器，左眼使用细胞刷。由2名观察者对经瑞氏-吉姆萨染色的标本进行评估。用4级评分法对细胞数量、保存情况、细胞形态以及杯状细胞和炎性细胞的存在情况进行评分。分析观察者间的一致性以及表面麻醉的效果。
在20份犬IC样本中，10份显示细胞数量良好（评分2 - 3），13份保存良好。13/20的IC样本中存在浅表上皮细胞（SEC），14/20中可见基底-中间细胞（BIC）。分别在6/20和7/20的样本中发现杯状细胞和炎性细胞。在20只猫中，15份IC样本细胞数量良好，14份保存良好，16/20 的IC样本中有SEC，17/20中有BIC。分别在13/20和3/20的猫样本中发现杯状细胞和炎性细胞。犬细胞刷标本显示保存良好（9/20）且细胞数量良好（8/20）。在猫细胞刷标本中，分别有16/20和14/20观察到保存良好和细胞数量良好。在两个物种中，所有细胞类型均存在，且没有明显分离。观察者之间对IC中细胞形态的一致性为中等至尚可。有无麻醉获取的标本具有可比性。
印迹细胞学检查能够采集到细胞结构得以保留的样本，而细胞质和细胞核细节往往难以评估。
