Wei Xiaoqin, Wang Yugang, Luo Runbo, Qian Wei, Sizhu Suolang, Zhou Hongbo
Department of Animal Science, Tibet Agricultural and Animal Husbandry College, Linzhi 860000, PR China; State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
Dev Comp Immunol. 2017 Jun;71:1-7. doi: 10.1016/j.dci.2017.01.017. Epub 2017 Jan 23.
The Pasteurella multocida lipoprotein B (PlpB) was cloned from Pasteurella multocida (P. multocida) strain LZ-PM (serotype A) and expressed in Escherichia coli (E. coli). Sequence analysis showed that PlpB from different strains of P. multocida exhibited 80.8-99.4% sequence identity to each other, suggesting that PlpB might serve as a cross-protective antigen. The purified PCR product of PlpB gene consisting of 831 base pairs was inserted into the pET-32a (+) plasmid, and then transferred into E. coli. The protective immunity conferred by recombinant PlpB (rPlpB) on mice was evaluated. The results showed that mice immunized with 200 μg of purified rPlpB were protected (60% survival rate) against challenge infection with 1 MLD of P. multocida strain LZ-PM. In conclusion, our data indicated that the PlpB protein may be a potential target as a candidate subunit vaccine for P. multocida infection.
多杀性巴氏杆菌脂蛋白B（PlpB）从多杀性巴氏杆菌（P. multocida）LZ-PM菌株（血清型A）中克隆出来，并在大肠杆菌（E. coli）中表达。序列分析表明，来自不同多杀性巴氏杆菌菌株的PlpB彼此之间的序列同一性为80.8%-99.4%，这表明PlpB可能作为一种交叉保护性抗原。将由831个碱基对组成的PlpB基因的纯化PCR产物插入pET-32a(+)质粒，然后转入大肠杆菌。评估了重组PlpB（rPlpB）对小鼠的保护性免疫。结果表明，用200μg纯化的rPlpB免疫的小鼠受到保护（存活率60%），免受1个最小致死剂量的多杀性巴氏杆菌LZ-PM菌株的攻击感染。总之，我们的数据表明，PlpB蛋白可能是作为多杀性巴氏杆菌感染候选亚单位疫苗的潜在靶点。
