Yu Xin-Zhe, Guo Zhong-Yi, Di Yang, Yang Feng, Ouyang Qi, Fu De-Liang, Jin Chen
Department of Pancreatic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
Hepatobiliary Pancreat Dis Int. 2017 Feb;16(1):104-109. doi: 10.1016/s1499-3872(16)60168-6.
Previous researches in pancreatic cancer demonstrated a negative correlation between secreted protein acidic and rich in cysteine (SPARC) expression in primary tumor and survival, but not for SPARC expression in lymph node. In the present study, we aimed to evaluate the SPARC expression in various types of tissues and its impact on patients' prognosis.
The expression of SPARC was examined by immunohistochemistry in resected pancreatic cancer specimens. Kaplan-Meier analyses and Cox proportional hazards regression were applied to assess the mortality risk.
A total of 222 tissue samples from 73 patients were collected to evaluate the SPARC expression, which included 73 paired primary tumor and adjacent normal tissues, 38 paired metastatic and normal lymph nodes. The proportion of positive SPARC expression in metastatic lymph node was high (32/38), whereas in normal lymph node it was negative (0/38). Positive SPARC expression in primary tumor cells was associated with a significantly decreased overall survival (P=0.007) and disease-free survival (P=0.003), whereas in other types of tissues it did not show a predictive role for prognosis. Univariate and multivariate analyses both confirmed this significance.
SPARC can serve a dual function role as both predictor for prognosis and potentially biomarker for lymph node metastasis in resected pancreatic cancer patients.
先前关于胰腺癌的研究表明，原发性肿瘤中富含半胱氨酸的酸性分泌蛋白（SPARC）的表达与生存率呈负相关，但在淋巴结中SPARC的表达与生存率无关。在本研究中，我们旨在评估SPARC在各种组织类型中的表达及其对患者预后的影响。
通过免疫组织化学检测切除的胰腺癌标本中SPARC的表达。应用Kaplan-Meier分析和Cox比例风险回归评估死亡风险。
共收集了73例患者的222个组织样本以评估SPARC的表达，其中包括73对原发性肿瘤和癌旁正常组织、38对转移淋巴结和正常淋巴结。转移淋巴结中SPARC阳性表达的比例较高（32/38），而正常淋巴结中均为阴性（0/38）。原发性肿瘤细胞中SPARC阳性表达与总生存期（P=0.007）和无病生存期（P=0.003）显著降低相关，而在其他组织类型中，其对预后未显示出预测作用。单因素和多因素分析均证实了这一显著性。
SPARC在接受手术切除的胰腺癌患者中可作为预后预测指标和潜在的淋巴结转移生物标志物，具有双重作用。
