Farid Hoda A, Morsy Zakariya S, Helmy Hanan, Ramzy Reda M R, El Setouhy Maged, Weil Gary J
Research and Training Center on Vectors of Diseases, Ain Shams University, Cairo, Egypt.
Am J Trop Med Hyg. 2007 Oct;77(4):593-600.
We used molecular xenomonitoring (MX, detection of filarial DNA in mosquitoes) to evaluate the impact of mass drug administration (MDA) in sentinel locations in Egypt with high (11.5%) and low (4.1%) baseline microfilaria prevalence rates. Blood-fed Culex pipiens were pooled by household and tested for Wuchereria bancrofti DNA by PCR. There was no significant relationship between the infection status of household residents and parasite DNA status of mosquitoes from the same houses. After 5 MDA rounds, parasite DNA rates in mosquitoes in high- and low-prevalence areas were reduced by 93.8% and 100% to 0.19% (95% CI: 0.076-0.382%) and 0% (95% CI: 0-0.045%), respectively. These changes were consistent with decreases in microfilaria prevalence rates in these sites; they provide insight regarding the minimal mosquito DNA rates necessary for sustained transmission of filariasis in Egypt. We conclude that MX is a powerful tool for monitoring the impact of MDA on filariasis endemicity and transmission.
我们采用分子异体监测（MX，即检测蚊子体内的丝虫DNA）来评估群体服药（MDA）对埃及高（11.5%）、低（4.1%）基线微丝蚴流行率的哨点地区的影响。按家庭将吸食人血的致倦库蚊汇集在一起，并通过聚合酶链反应检测班氏吴策线虫DNA。家庭居民的感染状况与来自同一房屋的蚊子的寄生虫DNA状况之间没有显著关系。经过5轮群体服药后，高流行区和低流行区蚊子体内的寄生虫DNA率分别降低了93.8%和100%，降至0.19%（95%置信区间：0.076 - 0.382%）和0%（95%置信区间：0 - 0.045%）。这些变化与这些地点微丝蚴流行率的下降一致；它们为埃及丝虫病持续传播所需的最低蚊子DNA率提供了见解。我们得出结论，分子异体监测是监测群体服药对丝虫病流行和传播影响的有力工具。
