Marei Hany E, Elnegiry Ahmed A, Zaghloul Adel, Althani Asma, Afifi Nahla, Abd-Elmaksoud Ahmed, Farag Amany, Lashen Samah, Rezk Shymaa, Shouman Zeinab, Cenciarelli Carlo, Hasan Anwarul
Biomedical Research Center, Qatar University, Doha, Qatar.
Department of Cytology and Histology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.
J Cell Physiol. 2017 Dec;232(12):3586-3597. doi: 10.1002/jcp.25826. Epub 2017 May 18.
Neural stem cells (NSCs) are multipotent self-renewing cells that could be used in cellular-based therapy for a wide variety of neurodegenerative diseases including Alzheimer's diseases (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), and multiple sclerosis (MS). Being multipotent in nature, they are practically capable of giving rise to major cell types of the nervous tissue including neurons, astrocytes, and oligodendrocytes. This is in marked contrast to neural progenitor cells which are committed to a specific lineage fate. In previous studies, we have demonstrated the ability of NSCs isolated from human olfactory bulb (OB) to survive, proliferate, differentiate, and restore cognitive and motor deficits associated with AD, and PD rat models, respectively. The use of carbon nanotubes (CNTs) to enhance the survivability and differentiation potential of NSCs following their in vivo engraftment have been recently suggested. Here, in order to assess the ability of CNTs to enhance the therapeutic potential of human OBNSCs for restoring cognitive deficits and neurodegenerative lesions, we co-engrafted CNTs and human OBNSCs in TMT-neurodegeneration rat model. The present study revealed that engrafted human OBNSCS-CNTs restored cognitive deficits, and neurodegenerative changes associated with TMT-induced rat neurodegeneration model. Moreover, the CNTs seemed to provide a support for engrafted OBNSCs, with increasing their tendency to differentiate into neurons rather than into glia cells. The present study indicate the marked ability of CNTs to enhance the therapeutic potential of human OBNSCs which qualify this novel therapeutic paradigm as a promising candidate for cell-based therapy of different neurodegenerative diseases.
神经干细胞（NSCs）是多能自我更新细胞，可用于多种神经退行性疾病的细胞治疗，包括阿尔茨海默病（AD）、帕金森病（PD）、肌萎缩侧索硬化症（ALS）和多发性硬化症（MS）。由于其本质上具有多能性，它们实际上能够分化为神经组织的主要细胞类型，包括神经元、星形胶质细胞和少突胶质细胞。这与定向分化为特定谱系命运的神经祖细胞形成显著对比。在先前的研究中，我们已经证明从人嗅球（OB）分离的神经干细胞分别具有在AD和PD大鼠模型中存活、增殖、分化以及恢复认知和运动功能缺陷的能力。最近有人提出使用碳纳米管（CNTs）来提高神经干细胞在体内移植后的存活率和分化潜能。在此，为了评估碳纳米管增强人嗅球神经干细胞恢复认知缺陷和神经退行性病变的治疗潜力的能力，我们在TMT - 神经退行性大鼠模型中共移植了碳纳米管和人嗅球神经干细胞。本研究表明，移植的人嗅球神经干细胞 - 碳纳米管恢复了与TMT诱导的大鼠神经退行性模型相关的认知缺陷和神经退行性变化。此外，碳纳米管似乎为移植的嗅球神经干细胞提供了支持，增加了它们分化为神经元而不是胶质细胞的倾向。本研究表明碳纳米管具有显著增强人嗅球神经干细胞治疗潜力的能力，这使这种新型治疗模式成为不同神经退行性疾病细胞治疗的有希望的候选者。
