Hesamizadeh Khashayar, Alavian Seyed Moayed, Najafi Tireh Shabankareh Azar, Sharafi Heidar
Baqiyatallah Research Center for Gastroenterology and Liver Diseases (BRCGL), Baqiyatallah University of Medical Sciences, Tehran, IR Iran; Middle East Liver Diseases (MELD) Center, Tehran, IR Iran.
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR Iran.
Hepat Mon. 2016 Nov 19;16(12):e42938. doi: 10.5812/hepatmon.42938. eCollection 2016 Dec.
Hepatitis C virus (HCV) is characterized by a high degree of genetic heterogeneity and classified into 7 genotypes and different subtypes. It heterogeneously distributed through various risk groups and geographical regions. A well-established phylogenetic relationship can simplify the tracing of HCV hierarchical strata into geographical regions. The current study aimed to find genetic phylogeny of subtypes 1a and 1b of HCV isolates based on NS5B nucleotide sequences in Iran and other members of Eastern Mediterranean regional office of world health organization, as well as other Middle Eastern countries, with a systematic review of available published and unpublished studies.
The phylogenetic analyses were performed based on the nucleotide sequences of NS5B gene of HCV genotype 1 (HCV-1), which were registered in the GenBank database. The literature review was performed in two steps: 1) searching studies evaluating the NS5B sequences of HCV-1, on PubMed, Scopus, and Web of Science, and 2) Searching sequences of unpublished studies registered in the GenBank database.
In this study, 442 sequences from HCV-1a and 232 from HCV-1b underwent phylogenetic analysis. Phylogenetic analysis of all sequences revealed different clusters in the phylogenetic trees. The results showed that the proportion of HCV-1a and -1b isolates from Iranian patients probably originated from domestic sources. Moreover, the HCV-1b isolates from Iranian patients may have similarities with the European ones.
In this study, phylogenetic reconstruction of HCV-1 sequences clearly indicated for molecular tracing and ancestral relationships of the HCV genotypes in Iran, and showed the likelihood of domestic origin for HCV-1a and various origin for HCV-1b.
丙型肝炎病毒（HCV）具有高度的基因异质性，可分为7个基因型和不同的亚型。它在不同的风险群体和地理区域中呈异质性分布。建立良好的系统发育关系可以简化HCV分层在地理区域中的追踪。本研究旨在基于伊朗以及世界卫生组织东地中海区域办事处的其他成员国和中东其他国家的NS5B核苷酸序列，对HCV分离株的1a和1b亚型进行基因系统发育分析，并对现有已发表和未发表的研究进行系统综述。
基于GenBank数据库中登记的HCV基因型1（HCV-1）的NS5B基因核苷酸序列进行系统发育分析。文献综述分两步进行：1）在PubMed、Scopus和Web of Science上搜索评估HCV-1的NS5B序列的研究，2）搜索GenBank数据库中登记的未发表研究的序列。
在本研究中，对442条HCV-1a序列和232条HCV-1b序列进行了系统发育分析。所有序列的系统发育分析在系统发育树中显示出不同的聚类。结果表明，伊朗患者的HCV-1a和-1b分离株比例可能源自国内。此外，伊朗患者的HCV-1b分离株可能与欧洲的分离株相似。
在本研究中，HCV-1序列的系统发育重建清楚地表明了伊朗HCV基因型的分子追踪和祖先关系，并显示了HCV-1a的国内起源可能性以及HCV-1b的多种起源可能性。
