IL28B基因附近多态性对丙型肝炎地中海贫血患者接受聚乙二醇干扰素和利巴韦林治疗反应的作用
The Role of Polymorphisms Near the IL28B Gene on Response to Peg-Interferon and Ribavirin in Thalassemic Patients With Hepatitis C.
作者信息
Behnava Bita, Sharafi Heidar, Keshvari Maryam, Pouryasin Ali, Mehrnoush Leila, Salimi Shima, Karimi Elizee Pegah, Ghazimoghaddam Mehran, Alavian Seyed Moayed
机构信息
Iran Hepatitis Network, Tehran, IR Iran; Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran, IR Iran; Middle East Liver Diseases (MELD) Center, Tehran, IR Iran.
Iran Hepatitis Network, Tehran, IR Iran; Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran, IR Iran; Middle East Liver Diseases (MELD) Center, Tehran, IR Iran; Armin Pathobiology Laboratory, Tehran, IR Iran.
出版信息
Hepat Mon. 2016 Jan 23;16(1):e32703. doi: 10.5812/hepatmon.32703. eCollection 2016 Jan.
BACKGROUND
Hepatitis C Virus (HCV) is the major cause of liver failure in thalassemic patients. In these patients, iron overload and their comorbidities make difficulties during Pegylated-Interferon (PEG-IFN) and Ribavirin (RBV) therapy.
OBJECTIVES
We aimed to assess the impact of polymorphisms near the IL28B gene on virological response in HCV - infected thalassemic patients, who were treated with PEG-IFN and RBV.
PATIENTS AND METHODS
This cross - sectional study was conducted on 143 thalassemic patients with chronic hepatitis C, who were treated with a combination of PEG-IFN and RBV regimen. The rs12979860 and rs8099917 polymorphisms were assessed as the most common polymorphisms near the IL28B gene by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
RESULTS
The rate of sustained virological response (SVR) was significantly lower in thalassemic patients with HCV genotype-1 infection compared to patients with HCV genotype-3 infection. Among baseline predictors, rs12979860 and rs8099917 polymorphisms were found to be the only parameters associated with achievement of SVR in thalassemic patients with HCV genotype-1 infection however, there was no association between these polymorphisms and the rate of SVR in thalassemic patients with HCV genotype-3 infection.
CONCLUSIONS
In HCV genotype-1- infected thalassemic patients with rs12979860 CC genotype and without severe comorbidities, PEG-IFN and RBV combination therapy can be tried yet in those with rs12979860 CT/TT it may be reasonable to treat cases with new direct-acting antivirals.
背景
丙型肝炎病毒（HCV）是地中海贫血患者肝衰竭的主要原因。在这些患者中，铁过载及其合并症给聚乙二醇干扰素（PEG-IFN）和利巴韦林（RBV）治疗带来困难。
目的
我们旨在评估IL28B基因附近多态性对接受PEG-IFN和RBV治疗的HCV感染地中海贫血患者病毒学应答的影响。
患者和方法
本横断面研究对143例慢性丙型肝炎地中海贫血患者进行，这些患者接受了PEG-IFN和RBV联合治疗方案。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法评估rs12979860和rs8099917多态性，将其作为IL28B基因附近最常见的多态性。
结果
与HCV基因3型感染患者相比，HCV基因1型感染的地中海贫血患者持续病毒学应答（SVR）率显著更低。在基线预测因素中，rs12979860和rs8099917多态性被发现是与HCV基因1型感染的地中海贫血患者实现SVR相关的唯一参数，然而，这些多态性与HCV基因3型感染的地中海贫血患者的SVR率之间没有关联。
结论
在HCV基因1型感染且rs12979860为CC基因型且无严重合并症的地中海贫血患者中，可以尝试PEG-IFN和RBV联合治疗，但对于rs12979860为CT/TT基因型的患者，使用新型直接作用抗病毒药物治疗可能是合理的。
Zotero 插件