miR-520a-3p通过CCND1和CD44在乳腺癌中的抑制作用
Suppressing role of miR-520a-3p in breast cancer through CCND1 and CD44.
作者信息
Li Jun, Wei Juan, Mei Zhu, Yin Yongmei, Li Yongfei, Lu Mingjie, Jin Shidai
机构信息
Department of Medical Oncology, The First Affiliated Hospital with Nanjing Medical University Nanjing, P. R. China.
Department of Medical Oncology, The Second Affiliated Hospital with Southeast University Nanjing, P. R. China.
出版信息
Am J Transl Res. 2017 Jan 15;9(1):146-154. eCollection 2017.
Abstract
Recent studies show that many microRNAs (miRNAs) are found to play important roles in breast cancer, however, most of miRNAs are not investigated completely. In the present study, significant down-regulation of miR-520a-3p was found in the breast cancer tissues and cell lines. The restoration of miR-520a-3p expression in breast cancer cells could inhibit cell proliferation, migration and invasion. In addition, miR-520a-3p was able to induce breast cancer cell apoptosis. Luciferase assay was used to confirm that CCND1 and CD44 were the direct target genes of miR-520a-3p. The ectopic expression of miR-520a-3p repressed CCND1 and CD44 expression on post-transcriptional levels in breast cancer cells. This study suggests that miR-520a-3p may act as an optional method for breast cancer therapy.
摘要
最近的研究表明，许多微小RNA（miRNA）在乳腺癌中发挥重要作用，然而，大多数miRNA尚未得到充分研究。在本研究中，发现乳腺癌组织和细胞系中miR-520a-3p显著下调。恢复乳腺癌细胞中miR-520a-3p的表达可抑制细胞增殖、迁移和侵袭。此外，miR-520a-3p能够诱导乳腺癌细胞凋亡。荧光素酶报告基因实验用于证实CCND1和CD44是miR-520a-3p的直接靶基因。miR-520a-3p的异位表达在转录后水平抑制乳腺癌细胞中CCND1和CD44的表达。本研究表明，miR-520a-3p可能成为乳腺癌治疗的一种选择方法。
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