Deichelbohrer Mona, Wu Ming-Feng, Seitz Berthold, Wagenpfeil Stefan, Meier Carola, Bischoff Markus, Tschernig Thomas
Institute of Anatomy and Cell Biology, Saarland University Faculty of Medicine, Homburg, D-66424 Saar, Germany.
Department of Ophthalmology, Saarland University Medical Center, Homburg, D-66424 Saar, Germany.
Biomed Rep. 2017 Jan;6(1):75-78. doi: 10.3892/br.2016.821. Epub 2016 Nov 30.
The successful treatment of severe bacterial keratitis continues to be a challenge in animals and humans. In the present study the aim was to assess gaseous therapy using gaseous nitric oxide (gNO) in a murine model of Pseudomonas aeruginosa keratitis. The cornea of anesthetized mice was mechanically scratched and covered with a bacterial suspension of . One day later, the infected eyes were exposed to 200 ppm NO for 30 min. Three to seven days later the mice were sacrificed and the bulbi were obtained and processed for light microscopy. The read out parameter was the maximal corneal thickness and the severity of the hypopyon. The therapy with NO did not result in either a reduction of inflammation concerning the maximal corneal thickness or the severity of the hypopyon. The bacterial load was not investigated due to technical limitations. Thus, exposure to gNO did not reduce the local inflammation in induced murine keratitis at the investigated time-points. This does not exclude effects of NO on the bacterial load, and in experimental and human keratitis.
严重细菌性角膜炎的成功治疗在动物和人类中仍然是一项挑战。在本研究中，目的是在铜绿假单胞菌角膜炎小鼠模型中评估使用气态一氧化氮（gNO）的气体疗法。麻醉小鼠的角膜被机械划伤并用细菌悬液覆盖。一天后，将受感染的眼睛暴露于200 ppm的NO中30分钟。三到七天后，处死小鼠并获取眼球并进行光学显微镜检查。读出参数为最大角膜厚度和前房积脓的严重程度。NO治疗并未导致关于最大角膜厚度或前房积脓严重程度的炎症减轻。由于技术限制，未对细菌载量进行研究。因此，在所研究的时间点，暴露于gNO并未减轻诱导的小鼠角膜炎中的局部炎症。这并不排除NO对细菌载量以及实验性和人类角膜炎的影响。
