Deichelbohrer Mona, Wu Ming-Feng, Seitz Berthold, Hüttenberger Dirk, Laschke Matthias W, Foth Hans-Jochen, Wonnenberg Bodo, Wagenpfeil Stefan, Meier Carola, Bischoff Markus, Tschernig Thomas
Institute of Anatomy and Cell Biology, Medical Faculty, Saarland University, D-66424 Homburg/Saar, Germany.
Department of Ophthalmology, Saarland University Medical Center/UKS, D-66424 Homburg/Saar, Germany.
Exp Ther Med. 2017 Nov;14(5):4509-4514. doi: 10.3892/etm.2017.5109. Epub 2017 Sep 5.
The successful treatment of bacterial keratitis remains an unsolved clinical problem. The current study aimed to establish a murine keratitis model and to investigate the effect of chlorin e6 (Ce6) and photodynamic inactivation (PDI) on corneal inflammation. The cornea of anesthetized mice was scratched and covered with a bacterial suspension of . A paste containing Ce6 was applied to the cornea with subsequent exposure to specified light. Two days later the animals were sacrificed, and the globes were processed for light microscopy. Evaluation parameters were the maximal corneal thickness and the severity of the hypopyon. The maximal corneal thickness of 290±16 µm in the infected and untreated group was significantly reduced to 220±8 µm in the infected and treated group (P<0.05). In addition, the hypopyon was less severe in the infected and treated group. In conclusion, the present study indicates that PDI using Ce6 may be a potential approach to treat patients suffering with severe bacterial keratitis.
细菌性角膜炎的成功治疗仍是一个未解决的临床问题。当前研究旨在建立小鼠角膜炎模型，并研究氯e6（Ce6）和光动力灭活（PDI）对角膜炎症的影响。将麻醉小鼠的角膜划伤，并用……的细菌悬液覆盖。将含Ce6的糊剂应用于角膜，随后暴露于特定光线下。两天后处死动物，将眼球进行处理以用于光学显微镜检查。评估参数为最大角膜厚度和前房积脓的严重程度。感染且未治疗组的最大角膜厚度为290±16 µm，在感染且治疗组中显著降低至220±8 µm（P<0.05）。此外，感染且治疗组的前房积脓不那么严重。总之，本研究表明使用Ce6的PDI可能是治疗重度细菌性角膜炎患者的一种潜在方法。
