硫内酯霉素的聚酮骨架由一种不同寻常的迭代聚酮合酶组装而成。
The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase.
作者信息
Yurkovich Marie E, Jenkins Robert, Sun Yuhui, Tosin Manuela, Leadlay Peter F
机构信息
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.
Department of Chemistry, University of Warwick, Library Road, CV4 7AL, UK.
出版信息
Chem Commun (Camb). 2017 Feb 9;53(13):2182-2185. doi: 10.1039/c6cc09934c.
Following the in vivo investigation of thiotetronate assembly in Lentzea sp. and in S. thiolactonus NRRL 15439 (Havemann et al., Chem. Commun., 2017, DOI: 10.1039/c6cc09933e), the minimal set of genes required for thiolactomycin production was determined through heterologous expression and the mechanism for polyketide assembly was established in vitro through incubation of recombinant TlmB with its substrates in the presence of either nonhydrolysable or hydrolysable chemical probes. The results presented here constitute unequivocal evidence of enzymatic processing by an unusual iterative polyketide synthase.
在对 Lentzea 菌属和硫内酯链霉菌 NRRL 15439 中硫特曲菌素组装进行体内研究之后（Havemann 等人，《化学通讯》，2017 年，DOI：10.1039/c6cc09933e），通过异源表达确定了硫内酯霉素产生所需的最小基因集，并通过在不可水解或可水解化学探针存在的情况下将重组 TlmB 与其底物一起孵育，在体外建立了聚酮化合物组装机制。此处呈现的结果构成了一种不寻常的迭代聚酮合酶进行酶促加工的确凿证据。
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