Krishna Priti, Prasad Bishun D, Rahman Tawhidur
School of Environmental and Rural Science, University of New England, Armidale, NSW, 2351, Australia.
Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bihar, 813210, India.
Methods Mol Biol. 2017;1564:193-202. doi: 10.1007/978-1-4939-6813-8_16.
Brassinosteroids (BRs) are a class of plant steroidal hormones that play essential roles in plant growth and development. Systematic studies had first been undertaken concomitantly to determine both the effects of exogenous BR on stress phenotypes of Arabidopsis thaliana and Brassica napus (rapeseed) seedlings and the expression of stress marker genes in BR-treated and untreated seedlings. When reproducible and convincing evidence of the role of BR in stress tolerance had been obtained, molecular mechanisms underlying the ability of BR to confer tolerance against heat, cold, drought, and salt stress, as well as pathogen resistance were studied with several molecular approaches and tools. The results of these studies have together provided valuable insights into how BRs, through their control of many basic cellular processes and stress responses, promote vigor in plants and prepare the plant to mount a dynamic response upon environmental challenges. Protocols to assess BR effects on abiotic stress tolerance in Arabidopsis and rapeseed seedlings are described here and they can be fine-tuned and adapted for other plant species.
油菜素甾醇（BRs）是一类植物甾体激素，在植物生长发育中发挥着重要作用。最初同时开展了系统研究，以确定外源BR对拟南芥和甘蓝型油菜（油菜）幼苗胁迫表型的影响，以及BR处理和未处理幼苗中胁迫标记基因的表达。当获得BR在胁迫耐受性中作用的可重复且有说服力的证据后，采用多种分子方法和工具研究了BR赋予耐热、耐寒、耐旱、耐盐胁迫能力以及抗病性的分子机制。这些研究结果共同为BR如何通过控制许多基本细胞过程和胁迫反应来促进植物活力以及使植物在环境挑战时做出动态反应提供了有价值的见解。本文描述了评估BR对拟南芥和油菜幼苗非生物胁迫耐受性影响的实验方案，这些方案可以进行微调并适用于其他植物物种。
