Karetin Yu, Pushchin I I
School of Natural Sciences, Department of Cell Biology and Genetics, Far Eastern Federal University, Russky Island, Ajax St., Laboratory building, L703, Vladivostok, Russia.
A.V. Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Palchevskogo St., 17, Vladivostok, Russia, 690041.
Protoplasma. 2017 Jul;254(4):1805-1811. doi: 10.1007/s00709-017-1078-z. Epub 2017 Jan 26.
A description and formal classification of in vitro spreading coelomocytes from the Aphelasterias japonica sea star was performed using 39 parameters of linear and nonlinear morphometry based on the correlation, factor, and cluster analysis. The comparison of a variety of clustering models revealed the optimum classification parameters and algorithms. As a result, four morphological types of spreading cells significantly differing in a number of structural parameters were identified. This approach may be an important alternative or addition to classical methods of classification of polymorphic, irregularly shaped cells, in particular, cell elements of the invertebrate immune system. It provides the optimum methodology for structural analysis and classification of cells as a part of their further investigation in terms of structure, function, ontogeny, and diversity.
基于相关性、因子和聚类分析，使用39个线性和非线性形态测量参数对日本海星体外铺展的体腔细胞进行了描述和正式分类。对各种聚类模型的比较揭示了最佳分类参数和算法。结果，鉴定出四种在许多结构参数上有显著差异的铺展细胞形态类型。这种方法可能是对多态性、形状不规则细胞（特别是无脊椎动物免疫系统的细胞成分）经典分类方法的重要补充或替代。它为细胞的结构分析和分类提供了最佳方法，以便在结构、功能、个体发育和多样性方面对其进行进一步研究。
