Basbouss-Serhal Isabelle, Pateyron Stéphanie, Cochet Françoise, Leymarie Juliette, Bailly Christophe
Sorbonne Universités, UPMC Université Paris 06, Centre National de la Recherche Scientifique, Institut de Biologie Paris-Seine, Unité Mixte de Recherche 7622, Biologie du Développement, F-75005 Paris, France (I.B.-S., F.C., J.L., C.B.).
Institute of Plant Sciences Paris Saclay IPS2, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Sud, Université Evry, Université Paris-Saclay, Bâtiment 630, 91405 Orsay, France (S.P.); and.
Plant Physiol. 2017 Mar;173(3):1709-1723. doi: 10.1104/pp.16.01933. Epub 2017 Jan 26.
The regulation of plant gene expression, necessary for development and adaptive responses, relies not only on RNA transcription but also on messenger RNA (mRNA) fate. To understand whether seed germination relies on the degradation of specific subsets of mRNA, we investigated whether the 5' to 3' RNA decay machinery participated in the regulation of this process. Arabidopsis () seeds of () and () mutants displayed distinct dormancy phenotypes. Transcriptome analysis and mutant seeds allowed us to identify genes that are likely to play a role in the control of germination. Study of 5' untranslated region features of these transcripts revealed that specific motifs, secondary energy, and GC content could play a role in their degradation by XRN4 and VCS, and Gene Ontology clustering revealed novel actors of seed dormancy and germination. Several specific transcripts identified as being putative targets of XRN4 and VCS in seeds (, , , and ) were further studied by reverse genetics, and their functional roles in the germination process were confirmed by mutant analysis. These findings suggest that completion of germination and its regulation by dormancy also depend on the degradation of specific subsets of mRNA.
植物基因表达的调控对于发育和适应性反应是必需的，它不仅依赖于RNA转录，还依赖于信使核糖核酸（mRNA）的命运。为了了解种子萌发是否依赖于特定mRNA子集的降解，我们研究了5'到3' RNA衰变机制是否参与了这一过程的调控。拟南芥（）的（）和（）突变体种子表现出不同的休眠表型。对（）和（）突变体种子的转录组分析使我们能够鉴定出可能在萌发控制中起作用的基因。对这些转录本5'非翻译区特征的研究表明，特定基序、二级能量和GC含量可能在它们被XRN4和VCS降解中发挥作用，基因本体聚类揭示了种子休眠和萌发的新参与者。通过反向遗传学进一步研究了几种被确定为种子中XRN4和VCS假定靶标的特定转录本（、、、和），并通过突变分析证实了它们在萌发过程中的功能作用。这些发现表明，萌发的完成及其通过休眠的调控也依赖于特定mRNA子集的降解。
