Nisari M, Yilmaz S, Eroz R, Ertekin T, Bircan D, Ulger H
Bratisl Lek Listy. 2017;118(1):61-65. doi: 10.4149/BLL_2017_012.
Curcumin is a polyphenol compound that has antioxidant, anticancer, anti-inflammatory, anti-hyperlipidemic and antimicrobial effects. Nucleolar-organizing regions are the sites of the gene on chromosomes. The present study was aimed to show the antitumoral effect of curcumin via AgNOR protein synthesis in Ehrlich's ascitic carcinoma (EAC) bearing mice.
Twenty three mice with EAC were randomly divided into 3 groups as positive control (n = 7), group 2 (n = 8) and 3 (n = 8) treated intraperitoneally with curcumin (25 mg/kg) and (50 mg/kg), respectively. The animals were sacrificed on Day 16, the solid tumors were removed out. Then, total AgNOR area/nuclear area (TAA/NA) and the mean AgNOR number were estimated for each mice.
Statistically significant differences were determined among the whole groups for TAA/NA ratio (p = 0.000), conversely mean AgNOR number (p = 0.361). When comparingthe two groups; while no difference was determined between the control and curcumin (25 mg/kg) groups (p = 0.061), the significant differences were detected between the control and curcumin (50 mg/kg) groups (p = 0.000) and between curcumin (25 mg/kg) and curcumin (50 mg/kg) groups (p = 0.000) for TAA/NA ratio. However, there was no significant difference for the mean AgNOR number in double comparison of the groups.
The current study showed that curcumin had a crucial function against cancer development. Also, both AgNOR values might be used as biomarkers for detection of the most reliable therapeutic dose selection of cancer treatment (Tab. 3, Fig. 2, Ref. 27).
姜黄素是一种具有抗氧化、抗癌、抗炎、抗高血脂和抗菌作用的多酚化合物。核仁组织区是染色体上基因的位点。本研究旨在通过检测荷艾氏腹水癌（EAC）小鼠中酸性非组蛋白银染核仁组织者区（AgNOR）蛋白的合成来显示姜黄素的抗肿瘤作用。
将23只荷EAC小鼠随机分为3组，阳性对照组（n = 7），第2组（n = 8）和第3组（n = 8），分别腹腔注射姜黄素（25 mg/kg）和（50 mg/kg）。在第16天处死动物，取出实体瘤。然后，估算每只小鼠的总AgNOR面积/核面积（TAA/NA）和平均AgNOR数量。
各组间TAA/NA比值差异有统计学意义（p = 0.000），相反，平均AgNOR数量差异无统计学意义（p = 0.361）。两组比较时；虽然对照组和姜黄素（25 mg/kg）组之间差异无统计学意义（p = 0.061），但对照组和姜黄素（50 mg/kg）组之间（p = 0.000）以及姜黄素（25 mg/kg）组和姜黄素（50 mg/kg）组之间（p = 0.000）的TAA/NA比值差异有统计学意义。然而，在各组的双比较中，平均AgNOR数量差异无统计学意义。
本研究表明姜黄素对癌症发展具有关键作用。此外，两种AgNOR值都可作为生物标志物，用于检测癌症治疗中最可靠的治疗剂量选择（表3，图2，参考文献27）。
