Schick T, Steinhauer M, Aslanidis A, Altay L, Karlstetter M, Langmann T, Kirschfink M, Fauser S
Department of Ophthalmology, University Hospital of Cologne, Cologne, Germany.
Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University Hospital of Cologne, Cologne, Germany.
Eye (Lond). 2017 May;31(5):810-813. doi: 10.1038/eye.2016.328. Epub 2017 Jan 27.
PurposeTo investigate complement activation in aqueous humor and in plasma of patients with neovascular age-related macular degeneration (nAMD).Patients and methodsAqueous humor and EDTA-plasma of 31 nAMD patients and 30 age-matched controls was collected. The levels of the complement factor 3 (C3), the regulators factor H (FH), and factor I (FI), and of the complement activation products Ba, C3a, and the terminal complement complex (sC5b-9) were measured. Associations between complement levels and phenotype were determined using Mann-Whitney U-test.ResultsIn plasma, no significant differences were found between the nAMD group and the control group. In aqueous humor, significantly increased levels of Ba (P=0.002), and C3a (P=0.002) indicate local complement activation in nAMD patients and a trend for a concomitant upregulation of the complement regulators FH (P=0.02) and FI (P=0.04).ConclusionsOur findings provide strong evidence for a local complement dysregulation in nAMD patients.
目的
研究新生血管性年龄相关性黄斑变性（nAMD）患者房水和血浆中的补体激活情况。
患者与方法
收集31例nAMD患者及30例年龄匹配的对照者的房水和乙二胺四乙酸（EDTA）抗凝血浆。检测补体因子3（C3）、调节因子H（FH）、因子I（FI）以及补体激活产物Ba、C3a和末端补体复合物（sC5b-9）的水平。采用曼-惠特尼U检验确定补体水平与表型之间的关联。
结果
在血浆中，nAMD组与对照组之间未发现显著差异。在房水中，Ba（P=0.002）和C3a（P=0.002）水平显著升高，表明nAMD患者存在局部补体激活，同时补体调节因子FH（P=0.02）和FI（P=0.04）有上调趋势。
结论
我们的研究结果为nAMD患者局部补体失调提供了有力证据。
