Department of Ophthalmology, Fukushima Medical University, Fukushima, Japan.
Department of Immunology, Fukushima Medical University, Fukushima, Japan.
Invest Ophthalmol Vis Sci. 2020 Nov 2;61(13):39. doi: 10.1167/iovs.61.13.39.
To investigate the characteristics of complement activation products and angiogenic cytokines in the aqueous humor in eyes with pachychoroid neovasculopathy (PNV) and neovascular age-related macular degeneration (nAMD).
This was a prospective, comparative, observational study. All patients with choroidal neovascularization were classified as PNV without polyps, PNV with polyps (polypoidal choroidal vasculopathy [PCV]), or drusen-associated nAMD according to the presence or absence of pachychoroid features and soft drusen. This study included a total of 105 eyes. Aqueous humor samples were collected from 25 eyes with PNV without polyps, 23 eyes with PCV, and 24 eyes with drusen-associated nAMD before intravitreal anti-vascular endothelial growth factor (VEGF) injection and cataract surgery in 33 control eyes. Clinical samples were measured for complement component 3a (C3a), C4a, C5a, VEGF, and macrophage chemoattractant protein 1 (MCP-1) using a bead-based immunoassay.
C3a and MCP-1 levels were significantly higher in PCV (P = 0.032 and P = 0.039, respectively) and drusen-associated nAMD (P = 0.01 for both comparisons) than in controls, and no difference was seen in C3a and MCP-1 levels between PNV and controls (P = 0.747 and P = 0.294, respectively). VEGF levels were significantly higher in PNV (P = 0.016), PCV (P = 0.009), and drusen-associated nAMD (P = 0.043) than in controls. In PNV, the VEGF levels elevated without elevated C3a and MCP-1.
PNV, PCV, and drusen-associated nAMD had significantly distinct profiles of complement activation products and cytokines in the aqueous humor.
研究厚脉络膜新生血管（PNV）和新生血管性年龄相关性黄斑变性（nAMD）眼房水中补体激活产物和血管生成细胞因子的特征。
这是一项前瞻性、对照、观察性研究。根据是否存在厚脉络膜特征和软性玻璃膜疣，将所有脉络膜新生血管患者分为无息肉 PNV、有息肉 PNV（息肉样脉络膜血管病变 [PCV]）和与玻璃膜疣相关的 nAMD。本研究共纳入 105 只眼。在 33 只对照眼中，25 只无息肉 PNV 眼、23 只 PCV 眼和 24 只与玻璃膜疣相关的 nAMD 眼在玻璃体腔内抗血管内皮生长因子（VEGF）注射和白内障手术前采集房水样本。使用基于珠的免疫测定法测量补体成分 3a（C3a）、C4a、C5a、VEGF 和巨噬细胞趋化蛋白 1（MCP-1）的临床样本。
PCV（P = 0.032 和 P = 0.039）和与玻璃膜疣相关的 nAMD（P = 0.01 用于两种比较）中的 C3a 和 MCP-1 水平显著高于对照组，PNV 与对照组之间的 C3a 和 MCP-1 水平无差异（P = 0.747 和 P = 0.294）。PNV（P = 0.016）、PCV（P = 0.009）和与玻璃膜疣相关的 nAMD（P = 0.043）中的 VEGF 水平明显高于对照组。在 PNV 中，VEGF 水平升高，而 C3a 和 MCP-1 水平没有升高。
PNV、PCV 和与玻璃膜疣相关的 nAMD 房水中补体激活产物和细胞因子的特征明显不同。
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