State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Immunology Department, Changzhi Medical College, Shanxi 046000, China.
Int J Antimicrob Agents. 2017 Mar;49(3):364-374. doi: 10.1016/j.ijantimicag.2016.10.024. Epub 2016 Dec 21.
To treat nontuberculous mycobacteria (NTM) infections more optimally, further research pertaining to mycobacterial susceptibility to antimicrobial agents is required. A total of 82 species of NTM reference strains and 23 species of NTM clinical isolates were included. Minimum inhibitory concentrations (MICs) for 41 drugs were determined using the microdilution method in cation-adjusted Mueller-Hinton broth. The results showed that most of the NTM were susceptible to aminoglycosides, quinolones, three macrolides (clarithromycin, azithromycin and roxithromycin), cefmetazole, linezolid and capreomycin. Rapidly growing mycobacterium strains were additionally susceptible to cefoxitin, clofazimine, rifapentine, doxycycline, minocycline, tigecycline, meropenem and sulfamethoxazole, whereas slowly growing mycobacterium strains were additionally susceptible to rifabutin. This study on the susceptibility of NTM includes the largest sample size of Chinese clinical isolates and reference strains. NTM species-specific drug susceptibility patterns suggested that it is urgent to identify the species of NTM, to normalise the treatment of NTM infectious disease and to clarify the resistance mechanisms of NTM.
为了更优化地治疗非结核分枝杆菌（NTM）感染，需要进一步研究分枝杆菌对抗菌药物的敏感性。共纳入 82 种 NTM 参考菌株和 23 种 NTM 临床分离株。采用阳离子调整 Mueller-Hinton 肉汤微量稀释法测定 41 种药物的最小抑菌浓度（MIC）。结果表明，大多数 NTM 对氨基糖苷类、喹诺酮类、三种大环内酯类（克拉霉素、阿奇霉素和罗红霉素）、头孢美唑、利奈唑胺和卷曲霉素敏感。快速生长分枝杆菌菌株还对头孢西丁、氯法齐明、利福喷汀、强力霉素、米诺环素、替加环素、美罗培南和磺胺甲噁唑敏感，而缓慢生长分枝杆菌菌株还对利福布汀敏感。这项关于 NTM 敏感性的研究包括了中国最大的临床分离株和参考菌株样本量。NTM 种特异性药物敏感性模式表明，迫切需要鉴定 NTM 的种类，规范 NTM 传染病的治疗，并阐明 NTM 的耐药机制。
