Derewenda Zygmunt S
Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, Virginia 22908-0836, USA.
Postepy Biochem. 2016;62(3):286-297.
Macromolecular X-ray crystallography has undergone a dramatic and astonishing transformation since its inception in mid 1950s, almost exclusively owing to the developments in three other fields: computer science; synchrotron radiation; and molecular biology. The process of structure solution from a single crystal, provided the quality of diffraction data is adequate, has been shortened from many years to hours, if not minutes. Yet, in spite of the exponential increase in the available structural information (~120, 000 structures in the Protein Data Bank today), many fundamental problems continue to be the subject of scientific controversy. This article contains personal recollections of the author, pertaining to two research projects - conducted nearly four decades apart - both of which touch upon such long standing discussion of the Monod-Wyman-Changeux theory of cooperativity (or 'conformational selection') vs the Koshland-Nemethy-Filmer theory of 'induced fit'. It is dedicated to Dr. Alexander Wlodawer on his 70th birthday, with best wishes of continuing success.
自20世纪50年代中期诞生以来，大分子X射线晶体学经历了巨大且惊人的变革，这几乎完全归功于其他三个领域的发展：计算机科学、同步辐射和分子生物学。如果衍射数据质量足够，从单晶解析结构的过程已从多年缩短至数小时，甚至数分钟。然而，尽管可用结构信息呈指数级增长（如今蛋白质数据库中有约120,000个结构），许多基本问题仍是科学争议的主题。本文包含作者的个人回忆，涉及两个相隔近四十年开展的研究项目，这两个项目都触及了关于协同性的莫诺-怀曼-尚热理论（或“构象选择”）与科什兰德-内梅西-菲尔默“诱导契合”理论的长期讨论。谨以此文献给亚历山大·弗洛达韦尔博士70岁生日，祝他继续取得成功。
