Song Woo Chul, Kim Kyoung-Ran, Park Miri, Lee Kyung Eun, Ahn Dae-Ro
The Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea.
Advanced Analysis Center, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea.
Biomater Sci. 2017 Feb 28;5(3):412-416. doi: 10.1039/c6bm00792a.
Spherical nucleic acids (SNAs) are spherically arranged oligonucleotides on core inorganic nanoparticles and have great potential for intracellular delivery of bioactive molecules, since they have been found to be internalized into mammalian cells. Understanding the factors that influence the cellular uptake of SNAs would be beneficial to design SNAs with novel uptake properties. We here report the effect of the sugar backbone type of the oligonucleotides on the cellular internalization of SNAs. After the preparation of SNAs with the oligonucleotides of five different sugar backbones, we analyze the cellular uptake efficiency quantitatively by flow cytometry and inductively coupled plasma mass spectrometry (ICP-MS). The data reveal that the uptake efficiencies and the uptake mechanisms significantly rely on the backbone type. These results suggest that the backbone modification can provide a unique handle to tune the cellular uptake behavior of SNAs.
球形核酸（SNA）是在核心无机纳米颗粒上呈球形排列的寡核苷酸，由于已发现它们可被哺乳动物细胞内化，因此在生物活性分子的细胞内递送方面具有巨大潜力。了解影响SNA细胞摄取的因素将有助于设计具有新型摄取特性的SNA。我们在此报告寡核苷酸的糖骨架类型对SNA细胞内化的影响。在用五种不同糖骨架的寡核苷酸制备SNA后，我们通过流式细胞术和电感耦合等离子体质谱法（ICP-MS）定量分析细胞摄取效率。数据表明，摄取效率和摄取机制显著依赖于骨架类型。这些结果表明，骨架修饰可为调节SNA的细胞摄取行为提供独特的方法。
