Institute of Nanostructure and Solid State Physics, University of Hamburg, Jungiusstraße 11c, Hamburg, Germany.
Center for Hybrid Nanostructures, University of Hamburg, Falkenried 88, Hamburg, Germany.
Sci Rep. 2017 Jan 30;7:41584. doi: 10.1038/srep41584.
We present a radio-frequency impedance-based biosensor embedded inside a semiconductor microtube for the in-flow detection of single cells. An impedance-matched tank circuit and a tight wrapping of the electrodes around the sensing region, which creates a close, leakage current-free contact between cells and electrodes, yields a high signal-to-noise ratio. We experimentally show a twofold improved sensitivity of our three-dimensional electrode structure to conventional planar electrodes and support these findings by finite element simulations. Finally, we report on the differentiation of polystyrene beads, primary mouse T lymphocytes and Jurkat T lymphocytes using our device.
我们提出了一种基于射频阻抗的生物传感器，将其嵌入半导体微管内，用于在流动状态下检测单细胞。阻抗匹配的槽路和电极在传感区域的紧密缠绕，在细胞和电极之间形成紧密、无漏电流的接触，从而获得了高信噪比。我们通过实验证明，我们的三维电极结构相对于传统的平面电极具有两倍的灵敏度提高，并通过有限元模拟支持了这些发现。最后，我们使用该装置报告了聚苯乙烯珠、原代小鼠 T 淋巴细胞和 Jurkat T 淋巴细胞的分化情况。
