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人唾液和牛唾液粘蛋白中唾液酸的比色分析

The colorimetric analysis of sialic acid in human saliva and bovine salivary mucin.

作者信息

Pearce E I, Major G N

出版信息

J Dent Res. 1978 Nov-Dec;57(11-12):995-1002. doi: 10.1177/00220345780570111701.

DOI:10.1177/00220345780570111701
PMID:281346
Abstract

Three colorimetric methods were compared for their suitability for the analysis of sialic acid in human mixed saliva and bovine submaxillary gland mucin. The thiobarbituric acid method was most precise with human saliva, appeared to be subject to the least interference and is therefore the method of choice for these samples. However, it is not possible to use this method for the analysis of bovine mucins. With these samples the resorcinol method was most suitable, being subject to the least interference and relatively precise. The direct Ehrlich method was least precise overall and gave high values with human whole saliva.

摘要

比较了三种比色法用于分析人混合唾液和牛下颌下腺粘蛋白中唾液酸的适用性。硫代巴比妥酸法对人唾液最为精确,似乎受到的干扰最小,因此是这些样品的首选方法。然而,无法使用该方法分析牛粘蛋白。对于这些样品,间苯二酚法最为适用,受到的干扰最小且相对精确。直接埃利希法总体上最不精确,对人全唾液给出的值较高。

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