Culling C F, Reid P E, Ramey C W, Dunn W L, Clay M G
Can J Biochem. 1977 Jul;55(7):778-92. doi: 10.1139/o77-114.
Various methods for the estimation of free and ketosidically bound sialic acid were investigated for accuracy and specificity. It was found that oxidation with 0.025 M sodium metaperiodate in pH 7.0 phosphate buffer at room temperature for 20 min provided a simple, rapid, sensitive method whereby both the free and the ketosidically bound acid present in a mixture could be quantitatively analyzed. On completion of the oxidation step, the bound sialic acid is estimated with resorcinol reagent and the free sialic acid with thiobarbituric acid reagent. Oxidation under these conditions permitted a facile analysis of mixtures of bovine submaxillary mucin and free N-acetylneuraminic acid, whereas the Warren thiobarbituric acid procedure gave an erroneous value for free sialic acid.
研究了多种用于估算游离和酮糖苷键结合唾液酸的方法的准确性和特异性。结果发现，在室温下于pH 7.0的磷酸盐缓冲液中用0.025 M偏高碘酸钠氧化20分钟，可提供一种简单、快速、灵敏的方法，借此能够对混合物中存在的游离和酮糖苷键结合的酸进行定量分析。氧化步骤完成后，用间苯二酚试剂估算结合的唾液酸，用硫代巴比妥酸试剂估算游离唾液酸。在这些条件下进行氧化，便于分析牛下颌粘蛋白和游离N-乙酰神经氨酸的混合物，而沃伦硫代巴比妥酸法对游离唾液酸给出的是错误值。
