Armengaud J, Duport C
CEA, DRF, IBiTec-S, SPI, Li2D, Laboratory "Innovative Technologies for Detection and Diagnostics", Bagnols-sur-Cèze, France.
SQPOV, UMR0408, Avignon Université, INRA, Avignon, France.
Methods Enzymol. 2017;586:211-227. doi: 10.1016/bs.mie.2016.09.033.
Pathogens are known to release in their environment a large range of toxins and other virulence factors. Their pathogenicity relies on this arsenal of exoproteins and their orchestrated release upon changing environmental conditions. Exoproteomics aims at describing and quantifying the proteins found outside of the cells, thus takes advantage of the most recent methodologies of next-generation proteomics. This approach has been applied with great success to a variety of pathogens increasing the fundamental knowledge on pathogenicity. In this chapter, we describe how the exoproteome should be prepared and handled for high-throughput identification of exoproteins and their quantitation by label-free shotgun proteomics. We also mentioned some bioinformatics tools for extracting information such as toxin similarity search.
已知病原体在其生存环境中会释放大量毒素和其他毒力因子。它们的致病性依赖于这些外泌蛋白库以及在环境条件变化时的有序释放。外泌蛋白质组学旨在描述和定量细胞外发现的蛋白质，因此利用了新一代蛋白质组学的最新方法。这种方法已成功应用于多种病原体，增加了对致病性的基础知识。在本章中，我们描述了应如何制备和处理外泌蛋白质组，以便通过无标记鸟枪法蛋白质组学对细胞外蛋白质进行高通量鉴定和定量。我们还提到了一些用于提取信息（如毒素相似性搜索）的生物信息学工具。
