Departamento de Microbiología y Parasitología, Facultad de Farmacia, Campus Sur 15782, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Marine Research Institute (IIM), Spanish National Research Council (CSIC), Eduardo Cabello 6, 36208 Vigo, Spain.
Int J Mol Sci. 2021 Jul 29;22(15):8141. doi: 10.3390/ijms22158141.
Some species are important human and animal pathogens that can be found in contaminated food and produce a variety of virulence factors involved in their pathogenicity. strains exhibiting multidrug resistance are known to be progressively increasing and that is why continuous monitoring is needed. Effective therapy against pathogenic requires identification of the bacterial strain involved, as well as determining its virulence factors, such as antibiotic resistance and sensitivity. The present study describes the use of liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) to do a global shotgun proteomics characterization for pathogenic species. This method allowed the identification of a total of 2990 non-redundant peptides, representing 2727 proteins. Furthermore, 395 of the peptides correspond to proteins that play a direct role in pathogenicity; they were identified as virulence factors, toxins and anti-toxins, or associated with either antibiotics (involved in antibiotic-related compounds production or resistance) or resistance to toxic substances. The proteomic repository obtained here can be the base for further research into pathogenic species and facilitate the development of novel therapeutics for these pathogens.
一些物种是重要的人类和动物病原体，可以在污染的食物中发现，并产生多种与致病性有关的毒力因子。具有多药耐药性的菌株逐渐增多，因此需要持续监测。针对致病性的有效治疗需要确定涉及的细菌菌株，并确定其毒力因子，如抗生素耐药性和敏感性。本研究描述了使用液相色谱-电喷雾电离串联质谱（LC-ESI-MS/MS）对致病性细菌物种进行全面的 shotgun 蛋白质组学特征描述。该方法共鉴定出 2990 个非冗余肽段，代表 2727 种蛋白质。此外，395 个肽段对应于在致病性中起直接作用的蛋白质；它们被鉴定为毒力因子、毒素和抗毒素，或与抗生素（涉及抗生素相关化合物的产生或耐药性）或对有毒物质的耐药性有关。这里获得的蛋白质组学数据库可以作为进一步研究致病性细菌物种的基础，并有助于开发针对这些病原体的新型治疗方法。
