Ma Yuewen, Li Qiulei, Hu Guibing, Qin Yonghua
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China, Ministry of Agriculture, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Gene. 2017 Apr 20;609:52-61. doi: 10.1016/j.gene.2017.01.033. Epub 2017 Jan 27.
Seedlessness is an excellent economical trait, and self-incompatibility (SI) is one of important factors resulting in seedless fruit in Citrus. However, SI molecular mechanism in Citrus is still unclear. In this study, RNA-Seq technology was used to identify differentially expressed genes related to SI reaction of 'Wuzishatangju' (Citrus reticulata Blanco). A total of 35.67GB raw RNA-Seq data was generated and was de novo assembled into 50,364 unigenes with an average length of 897bp and N50 value of 1549. Twenty-three candidate unigenes related to SI were analyzed using qPCR at different tissues and stages after self- and cross-pollination. Seven pollen S genes (Unigene0050323, Unigene0001060, Unigene0004230, Unigene0004222, Unigene0012037, Unigene0048889 and Unigene0004272), three pistil S genes (Unigene0019191, Unigene0040115, Unigene0036542) and three genes (Unigene0038751, Unigene0031435 and Unigene0029897) associated with the pathway of ubiquitin-mediated proteolysis were identified. Unigene0031435, Unigene0038751 and Unigene0029897 are probably involved in SI reaction of 'Wuzishatangju' based on expression analyses. The present study provides a new insight into the molecular mechanism of SI in Citrus at the transcriptional level.
无核是一种优良的经济性状，而自交不亲和性（SI）是导致柑橘类果实无核的重要因素之一。然而，柑橘中SI的分子机制仍不清楚。在本研究中，利用RNA-Seq技术鉴定了与‘无子砂糖橘’（Citrus reticulata Blanco）SI反应相关的差异表达基因。共产生了35.67GB的原始RNA-Seq数据，并将其从头组装成50364个单基因，平均长度为897bp，N50值为1549。使用qPCR对自花授粉和异花授粉后不同组织和阶段的23个与SI相关的候选单基因进行了分析。鉴定出7个花粉S基因（Unigene0050323、Unigene0001060、Unigene0004230、Unigene0004222、Unigene0012037、Unigene0048889和Unigene0004272）、3个雌蕊S基因（Unigene0019191、Unigene0040115、Unigene0036542）以及3个与泛素介导的蛋白水解途径相关的基因（Unigene0038751、Unigene0031435和Unigene0029897）。基于表达分析，Unigene0031435、Unigene0038751和Unigene0029897可能参与了‘无子砂糖橘’的SI反应。本研究在转录水平上为柑橘中SI的分子机制提供了新的见解。
