Williamson Chad R, Khurana Sandhya, Nguyen Phong, Byrne Collin J, Tai T C
Department of Biology, Laurentian University, Sudbury, ON, Canada.
Medical Sciences Division, Northern Ontario School of Medicine, Sudbury, ON, Canada.
Med Sci Monit Basic Res. 2017 Jan 31;23:20-24. doi: 10.12659/msmbr.901964.
BACKGROUND The renal renin-angiotensin system (RAS) is physiologically important for blood pressure regulation. Altered regulation of RAS-related genes has been observed in an animal model of hypertension (spontaneously hypertensive rats - SHRs). The current understanding of certain RAS-related gene expression differences between Wistar-Kyoto rats (WKYs) and SHRs is either limited or has not been compared. The purpose of this study was to compare the regulation of key RAS-related genes in the kidneys of adult WKYs and SHRs. MATERIAL AND METHODS Coronal sections were dissected through the hilus of kidneys from 16-week-old male WKYs and SHRs. RT-PCR analysis was performed for Ace, Ace2, Agt, Agtr1a, Agtr1b, Agtr2, Atp6ap2 (PRR), Mas1, Ren, Rnls, and Slc12a3 (NCC). RESULTS Increased mRNA expression was observed for Ace, Ace2, Agt, Agtr1a, Agtr1b, and Atp6ap2 in SHRs compared to WKYs. Mas1, Ren, Slc12a3, and Rnls showed no difference in expression between animal types. CONCLUSIONS This study shows that the upregulation of several key RAS-related genes in the kidney may account for the increased blood pressure of adult SHRs.
背景 肾脏肾素 - 血管紧张素系统（RAS）在血压调节中具有重要生理意义。在高血压动物模型（自发性高血压大鼠 - SHRs）中已观察到RAS相关基因调节的改变。目前对于Wistar - Kyoto大鼠（WKYs）和SHRs之间某些RAS相关基因表达差异的了解有限或尚未进行比较。本研究的目的是比较成年WKYs和SHRs肾脏中关键RAS相关基因的调节情况。
材料与方法 对16周龄雄性WKYs和SHRs的肾脏肾门处进行冠状切片。对Ace、Ace2、Agt、Agtr1a、Agtr1b、Agtr2、Atp6ap2（PRR）、Mas1、Ren、Rnls和Slc12a3（NCC）进行逆转录聚合酶链反应（RT-PCR）分析。
结果 与WKYs相比，SHRs中Ace、Ace2、Agt、Agtr1a、Agtr1b和Atp6ap2的mRNA表达增加。Mas1、Ren、Slc12a3和Rnls在两种动物类型之间的表达无差异。
结论 本研究表明，肾脏中几个关键RAS相关基因的上调可能是成年SHRs血压升高的原因。
