Department of Pharmacology, School of Medicine, Kyungpook National University, 680 GukchaeBosang Street, Daegu, 41944, Republic of Korea.
Cardiovascular Research Institute, Kyungpook National University, Daegu, 41944, Republic of Korea.
Sci Rep. 2024 Aug 29;14(1):20056. doi: 10.1038/s41598-024-69757-x.
Although Akkermansia muciniphila (Am) plays a beneficial role as a probiotic in the treatment of metabolic syndrome, the mechanisms remain elusive. We tested the hypothesis that Am extracellular vesicles (AmEVs) protect against hypertension through modulation of gene expression in the kidneys of spontaneously hypertensive rats (SHRs). Extracellular vesicles purified from anaerobically cultured Am (1.0 × 10 or 1.0 × 10 particles/kg) or vehicles were injected into the tail veins of Wistar-Kyoto rats (WKYs) and SHRs weekly for 4 weeks. Renal cortical tissues isolated from both rat strains were analyzed by trichrome stain and RT-qPCR. AmEVs protect against the development of hypertension in SHRs without a serious adverse reaction. AmEVs increased the expression of vasocontracting Agt and At1ar as well as vasodilating At2r, Mas1 and Nos2 in the kidneys of both strains. These results indicate that AmEVs have a protective effect against hypertension without a serious adverse reaction. Therefore, it is foreseen that AmEVs may be utilized as a novel therapeutic for the treatment of hypertension.
尽管黏蛋白阿克曼氏菌（Akkermansia muciniphila，Am）作为益生菌在代谢综合征的治疗中发挥有益作用，但具体机制仍不清楚。我们假设 Am 细胞外囊泡（AmEVs）通过调节自发性高血压大鼠（SHR）肾脏中的基因表达来预防高血压。从厌氧培养的 Am 中纯化的细胞外囊泡（1.0×10 或 1.0×10 个颗粒/公斤）或载体每周通过尾静脉注射到 Wistar-Kyoto 大鼠（WKYs）和 SHR 中 4 周。对来自两种大鼠品系的肾皮质组织进行三色染色和 RT-qPCR 分析。AmEVs 可预防 SHR 高血压的发生，且无严重不良反应。AmEVs 增加了两种大鼠肾脏中收缩血管的 Agt 和 At1ar 以及舒张血管的 At2r、Mas1 和 Nos2 的表达。这些结果表明，AmEVs 具有预防高血压的作用，且无严重不良反应。因此，预计 AmEVs 可作为治疗高血压的一种新型治疗方法。
