Université de Bordeaux, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
CNRS, UMR5797, Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Chemin du Solarium, 33175 Gradignan, France.
Sci Rep. 2017 Jan 31;7:41764. doi: 10.1038/srep41764.
As well as being a significant source of environmental radiation exposure, α-particles are increasingly considered for use in targeted radiation therapy. A better understanding of α-particle induced damage at the DNA scale can be achieved by following their tracks in real-time in targeted living cells. Focused α-particle microbeams can facilitate this but, due to their low energy (up to a few MeV) and limited range, α-particles detection, delivery, and follow-up observations of radiation-induced damage remain difficult. In this study, we developed a thin Boron-doped Nano-Crystalline Diamond membrane that allows reliable single α-particles detection and single cell irradiation with negligible beam scattering. The radiation-induced responses of single 3 MeV α-particles delivered with focused microbeam are visualized in situ over thirty minutes after irradiation by the accumulation of the GFP-tagged RNF8 protein at DNA damaged sites.
除了是环境辐射暴露的重要来源外，α 粒子也越来越多地被用于靶向放射治疗。通过实时跟踪靶向活细胞中的 α 粒子，可以更好地了解 DNA 水平上的 α 粒子诱导损伤。聚焦的 α 粒子微束可以促进这一点，但由于其能量低（高达几 MeV）且射程有限，α 粒子的检测、传递以及对辐射诱导损伤的后续观察仍然很困难。在这项研究中，我们开发了一种薄的掺硼纳米金刚石膜，允许可靠地检测单个 α 粒子并对单个细胞进行照射，同时几乎没有束散射。通过在照射后三十分钟内 GFP 标记的 RNF8 蛋白在 DNA 损伤部位的积累，对用聚焦微束输送的单个 3 MeV α 粒子的辐射诱导反应进行了原位可视化。
