University of Eastern Finland, Department of Environmental and Biological Sciences, Kuopio 70210, Finland.
Wageningen University, Laboratory of Genetics, Wageningen 6708 PB, The Netherlands.
Sci Data. 2017 Jan 31;4:160131. doi: 10.1038/sdata.2016.131.
Noccaea caerulescens of the Brassicaceae family has become the key model plant among the metal hyperaccumulator plants. Populations/accessions of N. caerulescens from geographic locations with different soil metal concentrations differ in their ability to hyperaccumulate and hypertolerate metals. Comparison of transcriptomes in several accessions provides candidates for detailed exploration of the mechanisms of metal accumulation and tolerance and local adaptation. This can have implications in the development of plants for phytoremediation and improved mineral nutrition. Transcriptomes from root and shoot tissues of four N. caerulescens accessions with contrasting Zn, Cd and Ni hyperaccumulation and tolerance traits were sequenced with Illumina Hiseq2000. Transcriptomes were assembled using the Trinity de novo assembler and were annotated and the protein sequences predicted. The comparison against the BUSCO plant early release dataset indicated high-quality assemblies. The predicted protein sequences have been clustered into ortholog groups with closely related species. The data serve as important reference sequences in whole transcriptome studies, in analyses of genetic differences between the accessions and other species, and for primer design.
十字花科蓝蓟属植物已成为金属超积累植物中的关键模式植物。来自土壤金属浓度不同地理区域的蓝蓟种群/品系在超积累和耐受金属方面的能力存在差异。对几个品系的转录组进行比较，为深入研究金属积累和耐受以及局部适应的机制提供了候选基因。这可能对开发用于植物修复和改善矿物质营养的植物具有重要意义。利用 Illumina Hiseq2000 对具有不同 Zn、Cd 和 Ni 超积累和耐受特性的 4 个蓝蓟品系的根和茎组织进行了转录组测序。使用 Trinity de novo 组装器对转录组进行组装，并对蛋白质序列进行预测。与 BUSCO 植物早期释放数据集的比较表明，组装质量很高。预测的蛋白质序列已聚类为与近缘物种的同源基因簇。这些数据可作为全转录组研究、品系间遗传差异分析以及引物设计的重要参考序列。
