University of Ljubljana, Biotechnical Faculty, Jamnikarjeva 101, SI-1000, Ljubljana, Slovenia.
Jožef Stefan Institute, Jamova 39, SI-1000, Ljubljana, Slovenia.
Sci Data. 2023 Dec 1;10(1):856. doi: 10.1038/s41597-023-02776-x.
Hyperaccumulators are a group of plant species that accumulate high concentrations of one or more metal(loid)s in their above-ground tissues without showing any signs of toxicity. Several hyperaccumulating species belong to the Brassicaceae family, among them the Cd and Zn hyperaccumulator Noccaea praecox. In this paper, we present de novo transcriptome assembled from two naturally occurring N. praecox populations growing in (i) metal-enriched soil and (ii) soil non-contaminated with metals (control site). Total RNA was extracted from the leaves of both populations. We obtained 801,935,101 reads, which were successfully assembled and annotated. The resulting assembly contains 135,323 transcripts, with 103,396 transcripts (76.4%) annotated with at least one function and encoding 53,142 putative proteins. Due to its close relationship with the hyperaccumulating model species N. cearulescens, it will be possible to derive protein functions from sequence comparisons with this species. Comparisons will highlight common and differing pathways of metal acquisition, storage, and detoxification which will allow us to expand our knowledge of these processes.
超积累植物是一类能够在地上部分组织中积累高浓度一种或多种金属（类金属）而不表现出任何毒性的植物物种。有几个超积累物种属于十字花科，其中 Cd 和 Zn 超积累植物弯叶遏蓝菜就是一个例子。在本文中，我们介绍了从生长在（i）富含金属的土壤和（ii）未受金属污染的土壤（对照点）中的两个自然发生的弯叶遏蓝菜种群中组装的从头转录组。从两个种群的叶片中提取总 RNA。我们获得了 801,935,101 条reads，成功地对其进行了组装和注释。所得组装包含 135,323 个转录本，其中 103,396 个转录本（76.4%）至少被注释了一个功能，并编码 53,142 个假定蛋白质。由于它与超积累模式物种 N. cearulescens 密切相关，因此可以通过与该物种的序列比较来推断蛋白质的功能。比较将突出金属吸收、储存和解毒的共同和不同途径，这将使我们能够扩展对这些过程的认识。
